PDF(Pubmed)
Abstract：
Osteosarcoma is a common primary malignancy of the bone. Osteosarcoma prognosis improves tremendously when chemotherapy is given in adjunct to surgical intervention. Limb reconstruction with sterilized autologous bone graft stabilized by orthopaedics implants has become a choice of treatment, but the biomechanical property of the bone treated with such sterilization method is a point of concern as a graft with inferior biomechanical property might lead to graft failure. This study compares the biomechanical properties of the bones treated with cryotherapy using liquid nitrogen and pasteurization in the form of four-point bending compression tests. Six sheep tibia bones were used for each group of treatment with one control group where no treatment was given. In the four-point bending test, osteotomy was performed at the tibia; the proximal tibia was treated with liquid nitrogen and pasteurization respectively. The treated bone is then reconstructed with the corresponding distal bone with locking plate and screws (Jiangsu Trauhiu Medical Instrument Co. Ltd., Changzhou, China). The four-point bending test was performed with an Electropulse® E3000 universal testing machine (Instron®, High Wycombe, United Kingdom) and results in the form of maximum compressive load, stress, and strain were collected. Photographic analysis of the fracture location and pattern were documented and analysed. We found that both methods of sterilization did not alter the biomechanical properties of the treated bone to the extent of statistical significance in comparison with the control group. However, other factors such as histological changes post treatment, equipment availability, and long-term outcome must be considered.
摘要：
骨肉瘤是骨的常见原发性恶性肿瘤。当在手术干预的辅助下进行化疗时，骨肉瘤的预后会大大提高。通过骨科植入物稳定的无菌自体骨移植进行肢体重建已成为治疗的选择，但是用这种灭菌方法处理的骨的生物力学特性是一个值得关注的问题，因为生物力学特性较差的移植物可能导致移植物失败。这项研究以四点弯曲压缩测试的形式比较了使用液氮和巴氏灭菌进行冷冻治疗的骨骼的生物力学特性。每个治疗组使用6只绵羊胫骨骨,其中一个对照组不给予治疗。在四点弯曲试验中,胫骨截骨;胫骨近端分别用液氮和巴氏灭菌法治疗。然后用锁定板和螺钉用相应的远端骨重建治疗骨(江苏Trauhiu医疗器械有限公司，常州,中国）。四点弯曲试验用Electropulse®E3000通用试验机(Instron®,HighWycombe,英国），并以最大压缩载荷的形式得出，压力，并收集菌株。记录并分析了断裂位置和图案的照相分析。我们发现，与对照组相比，两种灭菌方法均未改变治疗骨的生物力学特性至统计学意义。然而,其他因素，如治疗后的组织学变化，设备可用性,必须考虑长期结果。
