PDF(Pubmed)
Abstract：
Biliary calculi, a common benign disease of the gastrointestinal tract, are affected by multiple factors, including diet, lifestyle, living environment, and personal and genetic background. Its occurrence is believed to be related to a change in biliary microbiota. Approximately 10%-20% of symptomatic patients with cholecystolithiasis have choledocholithiasis, resulting in infection, abdominal pain, jaundice, and biliary pancreatitis. This study aimed to determine whether a dysfunction in the sphincter of Oddi, which controls the outflow of bile and separates the bile duct from the intestine, leads to a change in biliary microbiota and the occurrence of biliary calculi.
Forty patients with cholecystolithiasis and choledocholithiasis were prospectively recruited. Bile specimens were obtained, and biliary pressure was measured during and after surgery. The collected specimens were analyzed with 16S rRNA gene to characterize the biliary microbiota. The risk factors of common bile duct calculi were analyzed numerically combined with the pressure in the sphincter of Oddi.
Different biliary microbiota were found in all cases. Patients with sphincter of Oddi dysfunction had significantly increased biliary microbiota as well as significantly higher level of systemic inflammation than patients with normal sphincter of Oddi.
The systemic inflammatory response of patients with sphincter of Oddi dysfunction is more severe, and their microbial community significantly differs from that of patients with normal sphincter of Oddi, which makes biliary tract infection more likely; furthermore, the biliary tract of patients with sphincter of Oddi dysfunction has more gallstone-related bacterial communities.
Forty patients with cholecystolithiasis and choledocholithiasis were prospectively recruited. Bile specimens were obtained, and biliary pressure was measured during and after surgery. The collected specimens were analyzed with 16S rRNA gene to characterize the biliary microbiota. The risk factors of common bile duct calculi were analyzed numerically combined with the pressure in the sphincter of Oddi.
Different biliary microbiota were found in all cases. Patients with sphincter of Oddi dysfunction had significantly increased biliary microbiota as well as significantly higher level of systemic inflammation than patients with normal sphincter of Oddi.
The systemic inflammatory response of patients with sphincter of Oddi dysfunction is more severe, and their microbial community significantly differs from that of patients with normal sphincter of Oddi, which makes biliary tract infection more likely; furthermore, the biliary tract of patients with sphincter of Oddi dysfunction has more gallstone-related bacterial communities.
摘要：
未经批准：胆道结石，一种常见的胃肠道良性疾病，受到多种因素的影响，包括饮食,生活方式,生活环境,以及个人和遗传背景。它的发生被认为与胆道微生物群的变化有关。约10％-20％的有症状的胆囊结石患者有胆总管结石，导致感染，腹痛,黄疸,和胆源性胰腺炎.这项研究旨在确定Oddi括约肌是否功能障碍，它控制胆汁的流出并将胆管与肠道分开，导致胆道微生物群的变化和胆道结石的发生。
UNASSIGNED：前瞻性招募了40例胆囊结石和胆总管结石患者。获得胆汁标本，术中和术后测量胆道压力。用16SrRNA基因分析收集的标本以表征胆道微生物群。结合Oddi括约肌压力对胆总管结石的危险因素进行数值分析。
未经证实：在所有病例中均发现不同的胆道微生物群。Oddi括约肌功能异常的患者与Oddi括约肌正常的患者相比，胆道菌群明显增加，全身性炎症水平明显升高。
UASSIGNED：Oddi括约肌功能障碍患者的全身炎症反应更严重，它们的微生物群落与Oddi括约肌正常患者的微生物群落显著不同，这使得胆道感染的可能性更大；此外，Oddi括约肌功能障碍患者的胆道有更多与胆结石相关的细菌群落。
UNASSIGNED：前瞻性招募了40例胆囊结石和胆总管结石患者。获得胆汁标本，术中和术后测量胆道压力。用16SrRNA基因分析收集的标本以表征胆道微生物群。结合Oddi括约肌压力对胆总管结石的危险因素进行数值分析。
未经证实：在所有病例中均发现不同的胆道微生物群。Oddi括约肌功能异常的患者与Oddi括约肌正常的患者相比，胆道菌群明显增加，全身性炎症水平明显升高。
UASSIGNED：Oddi括约肌功能障碍患者的全身炎症反应更严重，它们的微生物群落与Oddi括约肌正常患者的微生物群落显著不同，这使得胆道感染的可能性更大；此外，Oddi括约肌功能障碍患者的胆道有更多与胆结石相关的细菌群落。
