PDF(Pubmed)
Abstract：
The development of biomaterials to enable application of antimicrobial peptides represents a strategy of high and current interest. In this study, a bioparticle was produced by the complexation between an antimicrobial polypeptide and the biocompatible and biodegradable polysaccharides chitosan-N-arginine and alginate, giving rise to a colloidal polyelectrolytic complex of pH-responsive properties. The inclusion of the polypeptide in the bioparticle structure largely increases the binding sites of complexation during the bioparticles production, leading to its effective incorporation. After lyophilization, detailed evaluation of colloidal structure of redispersed bioparticles evidenced nano or microparticles with size, polydispersity and zeta potential dependent on pH and ionic strength, and the dependence was not withdrawn with the polypeptide inclusion. Significant increase of pore edge tension in giant vesicles evidenced effective interaction of the polypeptide-bioparticle with lipid model membrane. Antibacterial activity against Aeromonas dhakensis was effective at 0.1% and equal for the isolated polypeptide and the same complexed in bioparticle, which opens perspectives to the composite material as an applicable antibacterial system.
摘要：
开发能够应用抗微生物肽的生物材料代表了当前高度关注的策略。在这项研究中,通过抗微生物多肽与生物相容性和可生物降解的多糖壳聚糖-N-精氨酸和藻酸盐之间的复合产生生物颗粒，产生具有pH响应特性的胶体聚电解质复合物。在生物颗粒结构中包含多肽极大地增加了生物颗粒生产过程中的复合结合位点。导致其有效合并。冻干后，详细评估再分散的生物颗粒的胶体结构证明纳米或微粒的大小，多分散性和ζ电位取决于pH和离子强度，并且在多肽包含的情况下没有消除依赖性。巨大囊泡中孔边缘张力的显着增加证明了多肽-生物颗粒与脂质模型膜的有效相互作用。对dhakensis气单胞菌的抗菌活性为0.1％，对于分离的多肽和在生物颗粒中复合的多肽相同，这为复合材料作为适用的抗菌系统开辟了前景。
