Abstract：
Achyrocline satureioides (Lam.) DC extract-loaded nanoemulsions have demonstrated potential for wound healing, with promising effects on keratinocyte proliferation. We carried out the first in vivo investigation of the wound healing activity of a hydrogel containing A. satureioides extract-loaded nanoemulsions. We prepared hydrogels by adding the gelling agent (Carbopol® Ultrez) to extract-loaded nanoemulsions (~250 nm in diameter) obtained by spontaneous emulsification. The final flavonoid content in formulation was close to 1 mg/mL, as estimated by ultra-fast liquid chromatography. Permeation/retention studies using porcine ear skin showed that flavonoids reached deeper layers of pig ear skin when it was damaged (up to 3.2 µg/cm² in the dermis), but did not reach the Franz-type diffusion cell receptor fluid. For healing activity, we performed a dorsal wound model using Wistar rats, evaluating the lesion size, anti-inflammatory markers, oxidative damage, and histology. We found that extract-loaded formulations promoted wound healing by increasing angiogenesis by ~20%, reducing inflammation (tumor necrosis factor α) by ~35%, decreasing lipid damage, and improving the re-epithelialization process in lesions. In addition, there was an increase in the number of blood vessels and hair follicles for wounds treated with the formulation compared with the controls. Our findings indicate that the proposed formulation could be promising in the search for better quality healing and tissue reconstruction.
摘要：
赤霉素satureioides(Lam.)负载DC提取物的纳米乳液已经证明了伤口愈合的潜力，对角质形成细胞增殖具有有希望的作用。我们进行了含有负载有紫胶提取物的纳米乳液的水凝胶的伤口愈合活性的首次体内研究。我们通过将胶凝剂(Carbopol®Ultrez)添加到通过自发乳化获得的负载提取物的纳米乳液(直径〜250nm)中来制备水凝胶。制剂中的最终类黄酮含量接近1mg/mL，由超快速液相色谱法估计。使用猪耳朵皮肤的渗透/保留研究表明，类黄酮在猪耳朵皮肤受损时达到更深的层（真皮中最高达3.2µg/cm²），但未到达Franz型扩散细胞受体液。为了治疗活动，我们用Wistar大鼠制作了背侧伤口模型，评估病变大小，抗炎标志物，氧化损伤，和组织学。我们发现，加载提取物的制剂可通过增加约20％的血管生成来促进伤口愈合，减少炎症（肿瘤坏死因子α）约35％，减少脂质损伤，并改善病变的再上皮化过程。此外,与对照相比,用所述制剂治疗的伤口的血管和毛囊数量增加.我们的发现表明，所提出的配方在寻找更好的质量愈合和组织重建方面可能很有希望。
