Abstract：
In traditional Chinese medicine, soft-shelled turtle protein and peptides serve as a nutraceutical for prolonging the lifespan. However, their effects on anti-aging have not been clarified scientifically in vivo. This study aimed to determine whether soft-shelled turtle peptides (STP) could promote the lifespan and healthspan in Drosophila melanogaster and the underlying molecular mechanisms. Herein, STP supplementation prolonged the mean lifespan by 20.23% and 9.04% in males and females, respectively, delaying the aging accompanied by climbing ability decline, enhanced gut barrier integrity, and improved anti-oxidation, starvation, and heat stress abilities, while it did not change the daily food intake. Mechanistically, STP enhanced autophagy and decreased oxidative stress by downregulating the target of rapamycin (TOR) signaling pathway. In addition, 95.18% of peptides from the identified sequences in STP could exert potential inhibitory effects on TOR through hydrogen bonds, van der Walls, hydrophobic interactions, and electrostatic interactions. The current study could provide a theoretical basis for the full exploitation of soft-shelled turtle aging prevention.
摘要：
在中医中,软壳龟蛋白和肽可作为延长寿命的营养品。然而,它们在体内抗衰老方面的作用尚未得到科学的阐明。这项研究旨在确定软壳龟肽（STP）是否可以促进果蝇的寿命和健康以及潜在的分子机制。在这里，补充STP使男性和女性的平均寿命延长了20.23％和9.04％，分别,延缓衰老伴随着攀爬能力下降，增强肠道屏障完整性，和改进的抗氧化，饥饿,和热应激能力，虽然它并没有改变日常食物的摄入量。机械上,STP通过下调雷帕霉素（TOR）信号通路，增强自噬，降低氧化应激。此外,95.18%来自STP中鉴定序列的肽可以通过氢键对TOR产生潜在的抑制作用,VanderWalls,疏水相互作用，和静电相互作用。本研究可为全面开发甲鱼衰老防治提供理论依据。
