Abstract：
The chemical composition, content of cholesterol, retinol and α-tocopherol, and the total antioxidant capacity of different tissues from wild beavers were investigated. The total phenolic contents and free radical scavenging activity (DPPH and ABTS assays) were analysed spectrophotometrically, and fat-soluble vitamins were quantified using high-performance liquid chromatography. The type of tissue (skeletal muscle from loin and hind leg vs. adipose tissue from subcutaneous fat and tail) significantly affected content of all chemical components. The concentration of cholesterol was not related to total fat content. The retinol and α-tocopherol contents (µg/100 g) were significantly higher in the tail (13.0 and 391.2) and subcutaneous fat (12.2 and 371.3) compared to skeletal muscles (as an average 9.1 and 361.4). Among all tissues the tail showed significantly the highest values of DPPH (3.07 mM TE/100 g), ABTS (3.33 mM TE/100 g), and total phenolics (TPC, 543.7 mg GAE/100 g). The concentration of retinol was positively correlated with α-tocopherol (0.748, p < 0.001), and both vitamins were very strongly correlated with DPPH (0.858 and 0.886, p < 0.001), ABTS (0.894 and 0.851, p < 0.001), and TPC (0.666 and 0.913, p < 0.001). The principal component analysis proved that moisture, ash, and protein contents were representative for skeletal muscles, whereas, retinol, α-tocopherol, ABTS and DPPH accurately described the antioxidant capacity of tissue from the tail.
摘要：
化学成分,胆固醇含量，视黄醇和α-生育酚，研究了野生海狸不同组织的总抗氧化能力。用分光光度法分析总酚含量和自由基清除活性（DPPH和ABTS测定），和脂溶性维生素使用高效液相色谱法进行定量。组织的类型（骨骼肌从腰部和后腿与皮下脂肪和尾巴的脂肪组织）显着影响所有化学成分的含量。胆固醇的浓度与总脂肪含量无关。与骨骼肌（平均9.1和361.4）相比，尾巴（13.0和391.2）和皮下脂肪（12.2和371.3）中的视黄醇和α-生育酚含量（µg/100g）明显更高。在所有组织中，尾巴显示出DPPH的最高值（3.07mMTE/100g），ABTS（3.33mMTE/100g），和总酚(TPC，543.7mgGAE/100g)。视黄醇浓度与α-生育酚浓度呈正相关(0.748,p<0.001),两种维生素均与DPPH密切相关（0.858和0.886，p<0.001），ABTS(0.894和0.851，p<0.001)，和TPC（0.666和0.913，p<0.001）。主成分分析证明，水分，灰,蛋白质含量代表骨骼肌，然而,视黄醇,α-生育酚,ABTS和DPPH从尾部准确描述了组织的抗氧化能力。
