Abstract：
Mature and efficient tissue culture systems are already available for most japonica rice varieties (Oryza sativa ssp. geng). However, it remains challenging to regenerate the majority of indica rice varieties (Oryza sativa ssp. xian). In this study, quantitative trait loci (QTLs) associated with rice callus regeneration ability were identified based on the plant regeneration rate (PRR) and total green plant rate (TGPR) of the 93-11 × Nip recombinant inbred line population. Significant positive correlations were found between PRR and TGPR. A total of three QTLs (one for PRR and two for TGPR) were identified. qPRR3 (located on chromosome 3) was detected for both traits, which could explain 13.40% and 17.07% of the phenotypic variations of PRR and TGPR, respectively. Subsequently, the effect of qPRR3 on callus regeneration ability was validated by cryptographically tagged near-isogenic lines (NILs), and the QTL was narrowed to an interval of approximately 160 kb. The anatomical structure observation of the regenerated callus of the NILs revealed that qPRR3 can improve the callus regeneration ability by promoting the regeneration of shoots.
摘要：
对于大多数粳稻品种（Oryzasativassp。geng)。然而,再生大多数in稻品种仍然具有挑战性（Oryza＆amp;nbsp;sativassp。西安)。在这项研究中,根据93-11×Nip重组自交系种群的植株再生率（PRR）和总绿株率（TGPR），鉴定了与水稻愈伤组织再生能力相关的数量性状位点（QTL）。PRR与TGPR呈正相关。总共鉴定了三个QTL(一个用于PRR,两个用于TGPR)。qPRR3（位于3号染色体上）检测到这两个性状，这可以解释PRR和TGPR表型变异的13.40%和17.07%，分别。随后，通过密码标记的近等基因系（NIL）验证了qPRR3对愈伤组织再生能力的影响，QTL缩小到大约160kb的间隔。对NIL再生愈伤组织的解剖结构观察表明，qPRR3可以通过促进芽的再生来提高愈伤组织的再生能力。
