Abstract：
BACKGROUND: Staphylococcus aureus is one of the most frequently major mastitis pathogens that cause clinical and subclinical mastitis worldwide. Current antimicrobial treatments are usually ineffective, and the commercially available vaccines lack proven effectiveness. The immunological response elicited by the recombinant S. aureus-cure-associated proteins phosphoglycerate kinase (PGK), enolase (ENO), and elongation factor-G (EF-G) in combination with the granulocyte-macrophage colony-stimulating factor (GM-CSF) DNA vaccination was studied in this work.
METHODS: Here, twenty-three C57BL/6 mice were divided into four groups and vaccinated with: G1: none (control); G2: GM-CSF DNA plasmid DNA vaccine; G3: the combination of EF-G+ENO+PGK; and G4: the combinations of EF-G+ENO+PGK proteins plus GM-CSF plasmid DNA vaccine. After 44 days, spleen cells were collected for immunophenotyping and lymphocyte proliferation evaluation by flow cytometry upon S. aureus stimulus.
RESULTS: Immunization with the three S. aureus recombinant proteins alone resulted in a higher percentage of IL-17A+ cells among CD8+ T central memory cells, as well as the highest intensity of IL-17A production by overall lymphocytes indicating that the contribution of the combined lymphocyte populations is crucial to sustaining a type 3 cell immunity environment.
CONCLUSIONS: The immunization with three S. aureus-cure-associated recombinant proteins triggered type 3 immunity, which is a highly interesting path to pursue an effective bovine S. aureus mastitis vaccine.
METHODS: Here, twenty-three C57BL/6 mice were divided into four groups and vaccinated with: G1: none (control); G2: GM-CSF DNA plasmid DNA vaccine; G3: the combination of EF-G+ENO+PGK; and G4: the combinations of EF-G+ENO+PGK proteins plus GM-CSF plasmid DNA vaccine. After 44 days, spleen cells were collected for immunophenotyping and lymphocyte proliferation evaluation by flow cytometry upon S. aureus stimulus.
RESULTS: Immunization with the three S. aureus recombinant proteins alone resulted in a higher percentage of IL-17A+ cells among CD8+ T central memory cells, as well as the highest intensity of IL-17A production by overall lymphocytes indicating that the contribution of the combined lymphocyte populations is crucial to sustaining a type 3 cell immunity environment.
CONCLUSIONS: The immunization with three S. aureus-cure-associated recombinant proteins triggered type 3 immunity, which is a highly interesting path to pursue an effective bovine S. aureus mastitis vaccine.
摘要：
背景：金黄色葡萄球菌是引起全球临床和亚临床乳腺炎的最常见的主要乳腺炎病原体之一。目前的抗菌治疗通常是无效的，和市售疫苗缺乏证明的有效性。重组金黄色葡萄球菌治愈相关蛋白磷酸甘油酸激酶（PGK）引起的免疫反应，烯醇化酶(ENO),在这项工作中，研究了延伸因子G（EF-G）与粒细胞-巨噬细胞集落刺激因子（GM-CSF）DNA疫苗的组合。
方法：这里，将23只C57BL/6小鼠分为四组，并接种以下疫苗：G1：无（对照）；G2：GM-CSFDNA质粒DNA疫苗；G3：EF-GENOPGK的组合；G4：EF-GENOPGK蛋白加GM-CSF质粒DNA疫苗的组合。44天后,收集脾细胞用于在金黄色葡萄球菌刺激下通过流式细胞术进行免疫表型分析和淋巴细胞增殖评估。
结果：用三种金黄色葡萄球菌重组蛋白单独免疫导致CD8+T中枢记忆细胞中IL-17A+细胞的百分比更高，以及总体淋巴细胞产生IL-17A的最高强度表明，合并的淋巴细胞群体的贡献对于维持3型细胞免疫环境至关重要。
结论：用三种金黄色葡萄球菌治愈相关重组蛋白免疫可触发3型免疫，这是寻求有效的牛金黄色葡萄球菌乳腺炎疫苗的一条非常有趣的途径。
方法：这里，将23只C57BL/6小鼠分为四组，并接种以下疫苗：G1：无（对照）；G2：GM-CSFDNA质粒DNA疫苗；G3：EF-GENOPGK的组合；G4：EF-GENOPGK蛋白加GM-CSF质粒DNA疫苗的组合。44天后,收集脾细胞用于在金黄色葡萄球菌刺激下通过流式细胞术进行免疫表型分析和淋巴细胞增殖评估。
结果：用三种金黄色葡萄球菌重组蛋白单独免疫导致CD8+T中枢记忆细胞中IL-17A+细胞的百分比更高，以及总体淋巴细胞产生IL-17A的最高强度表明，合并的淋巴细胞群体的贡献对于维持3型细胞免疫环境至关重要。
结论：用三种金黄色葡萄球菌治愈相关重组蛋白免疫可触发3型免疫，这是寻求有效的牛金黄色葡萄球菌乳腺炎疫苗的一条非常有趣的途径。
