PDF(Pubmed)
Abstract：
Nowadays, food authentication is more and more required given its relevance in terms of quality and safety. The seafood market is heavily affected by mislabelling and fraudulent substitutions/adulterations, especially for processed food products such as canned food items, due to the loss of morphological features. This study aims to develop new assays based on DNA to identify fresh mackerel (Scomber spp.) and commercial products. A new primer pair was de novo designed on the 5S rRNA gene and non-transcribed spacer (NTS), identifying a DNA mini-barcoding region suitable for species identification of processed commercial products. Moreover, to offer a fast and low-cost analysis, a new assay based on recombinase polymerase amplification (RPA) was developed for the identification of fresh \'Sgombro\' (Scomber scombrus) and \'Lanzardo o Occhione\' (Scomber japonicus and Scomber colias), coupled with the lateral flow visualisation for the most expensive species (Scomber scombrus) identification. This innovative portable assay has great potential for supply chain traceability in the seafood market.
UNASSIGNED:
UNASSIGNED: The online version contains supplementary material available at 10.1007/s12161-022-02429-6.
UNASSIGNED:
UNASSIGNED: The online version contains supplementary material available at 10.1007/s12161-022-02429-6.
摘要：
如今,鉴于食品认证在质量和安全方面的相关性，它越来越需要。海鲜市场受到错误标签和欺诈性替换/掺假的严重影响,特别是加工食品，如罐头食品，由于形态特征的丧失。这项研究旨在开发基于DNA的新测定方法，以鉴定新鲜的鲭鱼（Scomberspp。)和商业产品。在5SrRNA基因和非转录间隔区（NTS）上重新设计了一个新的引物对，鉴定适用于加工的商业产品的物种鉴定的DNA迷你条形码区。此外，提供快速和低成本的分析，开发了一种基于重组酶聚合酶扩增（RPA）的新方法，用于鉴定新鲜的“Sgombro”（Sccomberscombrus）和“LanzardooOcchione”（Sccomberjaponicus和Sccombercolias），再加上最昂贵的物种（Scomberscombrus）识别的侧向流动可视化。这种创新的便携式检测方法在海鲜市场的供应链可追溯性方面具有巨大潜力。
UNASSIGNED：
UNASSIGNED：在线版本包含补充材料，可在10.1007/s12161-022-02429-6获得。
UNASSIGNED：
UNASSIGNED：在线版本包含补充材料，可在10.1007/s12161-022-02429-6获得。
