PDF(Pubmed)
Abstract：
Caliciviruses are ssRNA viruses that can infect a wide range of hosts, including birds. While several avian caliciviruses have been discovered, their taxonomy and host distribution are largely unknown. We molecularly characterized a novel calicivirus (trumpeter swan calicivirus: TruSCV) in trumpeter swans over-wintering in south-west British Columbia, Canada. The positivity rate was 20.3% (14/69) and there were no significant differences in infection rates between males (5/34, 14.7%) and females (9/35, 25.7%) or among considered age groups (juveniles: 4/14, 28.6%; sub-adults: 1/9, 11.1%; adults: 9/46, 19.6%). Twelve infected swans died of lead poisoning, one because of starvation, and one from physical injuries. TruSCV complete genome possessed the typical organization and protein motifs of caliciviruses and a type 2 IRES and its closest relative was a virus circulating in Australian ducks. Phylogenetic analyses showed the existence of 34 different but monophyletic avian caliciviruses. These viruses, while having conserved genomic organization and protein motifs, possess different IRES types and group in several divergent clades, with only two of them corresponding to currently defined genera, highlighting the need for epidemiological investigations and systematic analyses to better define their taxonomy. Follow-up studies are needed to elucidate the diversity, distribution, and pathogenic potential of TruSCV.
摘要：
杯状病毒是ssRNA病毒，可以感染多种宿主，包括鸟类。虽然已经发现了几种禽杯状病毒，它们的分类法和宿主分布在很大程度上是未知的。我们在不列颠哥伦比亚省西南部的小号天鹅越冬中对一种新型的杯状病毒（小号天鹅杯状病毒：TruSCV）进行了分子鉴定，加拿大。阳性率为20.3％（14/69），男性（5/34，14.7％）和女性（9/35，25.7％）或考虑的年龄组（青少年：4/14，28.6％；亚成人：1/9，11.1％；成年人：9/46，19.6％）之间的感染率无显着差异。十二只受感染的天鹅死于铅中毒，一个是因为饥饿，还有一个身体受伤。TruSCV完整基因组具有杯状病毒的典型组织和蛋白质基序以及2型IRES，其最接近的亲戚是在澳大利亚鸭子中传播的病毒。系统发育分析表明存在34种不同但单一的禽杯状病毒。这些病毒,虽然具有保守的基因组组织和蛋白质基序，在几个不同的分支中拥有不同的IRES类型和群体，其中只有两个对应于当前定义的属，强调需要进行流行病学调查和系统分析，以更好地定义其分类法。需要后续研究来阐明多样性，分布，和TruSCV的致病潜力。
