Abstract：
The present study was undertaken to evaluate the efficacy of 2 graded levels (0.03 and 0.05% of diet) of a monoglyceride blend containing butyric, caprylic, and capric acids in broilers\' diet for optimizing gut structure and animal growth performance. For this purpose, a total of 210, one-day-old male Ross 308 broiler chicks were randomly allocated to 3 experimental treatments using 7 replicates each and 10 birds/replicate. The treatment groups involved supplementation of blend of short and medium chain fatty acids at the level of 0, 0.03, and 0.05% of the diet for 42 d. The incorporation of mixes of monoglycerides into broilers\' diet linearly improved BWG between d 0 and 21 (P = 0.034). At the end of trial, however, no significant changes were observed in performance indexes (BWG, FI, FCR). Jejunal morphometric parameters (villus height, crypt depth, and their ratio) remained unaltered with the monoglyceride supplementation on d 21. The results further showed that monoglycerides supplementation increased the goblet cell counts along the jejunal villi (P = 0.034) and crypt regions (P = 0.022), as well as it effectively modulated the mRNA abundances of tight junction protein (ZO-1, P = 0.033) and nutrient transporters (SGLT, PePT1; P = 0.005, 0.023, respectively) in the jejunum. Moreover, the downregulation in mRNA abundance of TNFα (P = 0.030) was observed with the monoglyceride supplementation. The SCFAs analysis of cecal contents showed no notable differences with monoglyceride blend supplementation when compared to the unsupplemented group. Collectively, high goblet cell numbers in the jejunum along with downregulation of the mRNA abundances of pro-inflammatory cytokines, upregulation of tight junction proteins, and nutrient transporters showed favorable responses of low doses of monoglycerides blend in broiler feeding. Further studies should be conducted in different rearing conditions to examine the effectiveness of such low levels of a monoglyceride blend in the modulation of gut structure, its functionality and animal performance.
摘要：
本研究旨在评估含有丁酸的单甘油酯混合物的2个分级水平（饮食的0.03和0.05％）的功效，辛酸,和癸酸在肉仔鸡日粮中的作用，以优化肠道结构和动物生长性能。为此,将总共210只1日龄的雄性Ross308肉鸡随机分配到3个实验处理中,每个处理7个重复,每个重复10只鸟。治疗组包括以饮食的0、0.03和0.05％的水平补充短链脂肪酸和中链脂肪酸的混合物，持续42d。在第0天和第21天之间，将甘油单酯混合物掺入肉鸡饮食中线性改善了BWG（P=0.034）。审判结束时,然而,在性能指标(BWG，FI,FCR）。空肠形态参数(绒毛高度，地穴深度,以及它们的比例）在第21天补充单甘油酯时保持不变。结果进一步表明，单甘酯的补充增加了沿空肠绒毛（P=0.034）和隐窝区域（P=0.022）的杯状细胞计数，以及它有效地调节紧密连接蛋白（ZO-1，P=0.033）和营养转运蛋白（SGLT，空肠中的PePT1；分别为P=0.005、0.023）。此外，单甘油酯补充剂观察到TNFαmRNA丰度的下调(P=0.030)。与未补充组相比，盲肠内容物的SCFA分析显示，单甘油酯混合物补充没有显着差异。总的来说，空肠杯状细胞数量高以及促炎细胞因子mRNA丰度的下调，紧密连接蛋白的上调，和营养转运蛋白在肉鸡饲喂中对低剂量单甘酯混合物表现出良好的反应。应在不同的饲养条件下进行进一步的研究，以检查这种低水平的单甘油酯混合物在肠道结构调节中的有效性。其功能和动物性能。
