Abstract：
Imaging with radiolabeled exendin enables detection and characterization of glucagon-like peptide 1 receptors (GLP-1Rs) in vivo with high specificity. The novel radiotracer [68Ga]Ga-NODAGA-exendin-4 forms a stable complex after a simple and fast labeling procedure. Beta-cell mass in the islets of Langerhans can be visualized using [68Ga]Ga-NODAGA-exendin-4, which is promising for research into diabetes mellitus (DM) pathophysiology. Furthermore, this radiotracer enables very sensitive detection of insulinomas, resulting from vast overexpression of GLP-1Rs, and seems promising for the detection of focal lesions in congenital hyperinsulinism (CHI). Here, we describe the procedures involved in [68Ga]Ga-NODAGA-exendin-4 positron emission tomography (PET)/computed tomography (CT) imaging including the radiolabeling of the NODAGA-exendin conjugate with 68Ga, quality controls, and PET/CT.
摘要：
用放射性标记的exendin成像能够以高特异性在体内检测和表征胰高血糖素样肽1受体(GLP-1R)。新型放射性示踪剂[68Ga]Ga-NODAGA-exendin-4在简单而快速的标记程序后形成稳定的复合物。可以使用[68Ga]Ga-NODAGA-exendin-4可视化朗格汉斯胰岛中的β细胞团，这有望用于糖尿病（DM）病理生理学的研究。此外，这种放射性示踪剂能够非常灵敏地检测胰岛素瘤，由于GLP-1R的大量过表达，对于先天性高胰岛素血症（CHI）的局灶性病变的检测似乎很有希望。这里,我们描述了[68Ga]Ga-NODAGA-exendin-4正电子发射断层扫描（PET）/计算机断层扫描（CT）成像中涉及的程序，包括用68Ga放射性标记NODAGA-exendin缀合物，质量控制,PET/CT。
