Abstract：
This research examined the histological micro-structure of tendril vasculature in cucurbitaceous taxa. In this research, the tendril anatomy of 17 taxa of Cucurbitaceae categorized into seven genera, including Cucumis (five species), Cucurbita and Luffa (three species each), Citrullus and Momordica (two species each) while Lagenaria and Praecitrullus (one species each), collected from different areas of the Thal desert were examined via microscopic imaging to explore its taxonomic significance. Tendril transverse sections were cut with a Shandon Microtome to prepare slides. The distinctive characteristics of taxonomic value (qualitative and quantitative) include tendril and vascular bundle shape, variation in the number of vascular bundles, tendril diameter length, layers of sclerenchyma, and shape of collenchyma and epidermal cells. Tendril shapes observed are irregular, slightly oval-shaped, slightly C shaped, angular (4-angled, 6-angled, or polygonal), and star shaped. Quantitative measurements were taken to analyze the data statistically using SPSS software. Cucurbita pepo had a maximum tendril diameter length of 656.1 µm and a minimum in Momordica balsamina of 123.05 µm. The highest number of vascular bundles (12) were noticed in Luffa acutangula var.amara. Angular type was prominent in collenchyma, and irregular shape was dominant in sclerenchyma cells. A maximum of seven to nine sclerenchyma layers were present in Lagenaria siceraria and a minimum of two or three layers in Cucumis melo subsp. agrestis, Cucumis melo var. flexuosus, and Cucumis melo var.cantalupensis. Epidermis cells also show great variations with a rectangular shape being dominant. Statistical UPGMA dendrogram clustering of tendril vasculature traits shows that histological sections studied with microscopic techniques can be used to identify species and will play a vital role in future taxonomic and phylogenic linkages.
摘要：
这项研究检查了葫芦类群中卷须脉管系统的组织学微观结构。在这项研究中,葫芦科17个类群的卷须解剖，分为7个属，包括黄瓜（五种），葫芦和丝瓜（各三种），Citrullus和苦瓜（各两种），Lagenaria和Praecitrullus（各一种），通过显微成像检查了从塔尔沙漠不同地区收集的数据，以探讨其分类学意义。用Shandon切片机切割卷须横切面以制备载玻片。分类学价值(定性和定量)的显著特征包括卷须和维管束形,维管束数量的变化，卷须直径长度,层的sclelenchyma，以及结性腺和表皮细胞的形状。观察到的卷须形状不规则，略呈椭圆形,略呈C形,角(4角，6角,或多边形)，星形。使用SPSS软件进行定量测量以对数据进行统计学分析。西葫芦的最大卷须直径长度为656.1µm，苦瓜的最小值为123.05µm。在Luffaacutangulavar中发现了最高数量的维管束（12）。阿马拉。角型在结膜中突出，匀浆细胞以不规则形状为主。在Lagenariasiceraria中最多存在7到9个scleenchyma层，在Cucumismelo亚种中最少存在2或3层。agrestis,黄瓜甜瓜变种。屈肌,和黄瓜甜瓜。cantalupensis.表皮细胞也显示出很大的变化，矩形占主导地位。卷须脉管系统特征的统计UPGMA树状图聚类表明，用微观技术研究的组织学切片可用于鉴定物种，并将在未来的分类学和系统发育联系中起着至关重要的作用。
