Abstract：
AGAMOUS-like 15 (AGL15) is a member of the MADS-domain transcription factor (TF) family. MADS proteins are named for a conserved domain that was originally from an acronym derived from genes expressed in a variety of eukaryotes (MCM1-AGAMOUS-DEFICIENS-SERUM RESPONSE FACTOR). In plants, this family has expanded greatly, with more than one-hundred members generally found in dicots, and the proteins encoded by these genes have often been associated with developmental identity. AGL15 transcript and protein accumulate primarily in embryos and has been found to promote an important process called plant regeneration via somatic embryogenesis (SE). To understand how this TF performs this function, we have previously used microarray technologies to assess direct and indirect responsive targets of this TF. We have now revisited this question using next generation sequencing (NGS) to both characterize in vivo binding sites for AGL15 as well as response to the accumulation of AGL15. We compared these data to the prior microarray results to evaluate the different platforms. The new NGS data brought to light an interaction with brassinosteroid (BR) hormone signaling that was \"missed\" in prior Gene Ontology analysis from the microarray studies.
摘要：
AGAM0US样15(AGL15)是MADS结构域转录因子(TF)家族的成员。MADS蛋白以保守域命名,该保守域最初来源于多种真核生物(MCM1-AGAMOUS-缺陷-血清反应因子)中表达基因的首字母缩写词。在植物中,这个家庭大大扩张了，在双子叶中通常有一百多个成员，这些基因编码的蛋白质通常与发育特性有关。AGL15转录物和蛋白质主要在胚胎中积累，并已发现通过体细胞胚胎发生（SE）促进称为植物再生的重要过程。要了解此TF如何执行此功能，我们以前已经使用微阵列技术来评估这种TF的直接和间接响应靶标。我们现在已经使用下一代测序（NGS）重新审视了这个问题，以表征AGL15的体内结合位点以及对AGL15积累的反应。我们将这些数据与先前的微阵列结果进行比较以评估不同的平台。新的NGS数据揭示了与油菜素类固醇（BR）激素信号的相互作用，该相互作用在微阵列研究的先前基因本体论分析中被“遗漏”。
