METHODS: We describe the pathophysiology of pregnancy complications in a patient with cTTP using platelet procoagulant membrane dynamics analysis and quantitative proteomic studies, compared to four pregnant patients with gestational hypertension, four pregnant patients with preeclampsia, and four healthy pregnant controls.
RESULTS: The cTTP patient had increased P-selectin, tissue factor expression, annexin-V binding on platelets and neutrophils, and localized thrombin generation, suggestive of hypercoagulability. Among 15 proteins that were upregulated, S100A8 and S100A9 were distinctly overexpressed.
CONCLUSIONS: There is platelet-neutrophil activation and interaction, platelet hypercoagulability, and proinflammation in our case of cTTP with fetal demise.
方法:我们使用血小板促凝膜动力学分析和定量蛋白质组学研究描述了cTTP患者妊娠并发症的病理生理学,与四名妊娠高血压患者相比,四名先兆子痫孕妇,和四个健康的孕妇对照。
结果:cTTP患者P-选择素升高,组织因子表达,膜联蛋白V在血小板和中性粒细胞上的结合,和局部的凝血酶生成,提示高凝。在15种上调的蛋白质中,S100A8和S100A9明显过表达。
结论:存在血小板-中性粒细胞活化和相互作用,血小板高凝,在我们的cTTP伴胎儿死亡的病例中促炎症。