Abstract：
The OECD test guidelines for animal experiments play an important role in evaluating the chemical hazards. Animal tests performed using OECD guidelines, especially when the good laboratory practice (GLP) principle is applied, reduce the duplication of toxicity testing and ensure the best mutual acceptance of data by the OECD\'s Mutual Acceptance of Data (MAD). The OECD inhalation toxicity test guidelines 412 (28 days) and 413 (90 days) have been revised. These OECD guidelines now reflect the inclusion of nanomaterials and recent scientific and technological developments. In particular, these test guidelines aim to evaluate the bronchoalveolar lavage fluid in the lungs for objective toxicity evaluation, along with the existing subjective histopathological evaluation. For solid particles, the lung burden measurement of particles is required for toxicokinetic studies and, in order to properly perform a toxicokinetic study, two post-exposure observations are recommended. In light of the revised OECD guidelines, we propose a method to reduce the number of animals when testing is conducted for nanomaterials.
摘要：
OECD动物实验测试指南在评估化学危害方面发挥着重要作用。使用经合组织指南进行的动物试验，特别是当良好的实验室规范（GLP）原则被应用时，减少毒性测试的重复，并确保OECD相互接受数据（MAD）的最佳相互接受数据。经合组织吸入毒性试验准则412(28天)和413(90天)已经修订。这些经合组织的指导方针现在反映了纳米材料和最近的科学和技术发展。特别是,这些测试指南旨在评估肺部支气管肺泡灌洗液的客观毒性评估，以及现有的主观组织病理学评估。对于固体颗粒，毒物代谢动力学研究需要颗粒的肺负荷测量，为了正确地进行毒物动力学研究，建议进行两次曝光后观察.根据经修订的经合组织准则，我们提出了一种方法，以减少动物的数量时，进行测试的纳米材料。
