PDF(Pubmed)
Abstract：
Mycobacterium intracellulare is the most common cause of nontuberculous mycobacterial lung disease, with a rapidly growing prevalence worldwide. In this study, we performed comparative genomic analysis and antimicrobial susceptibility characteristics analysis of 117 clinical M. intracellulare strains in China. Phylogenetic analysis showed that clinical M. intracellulare strains had high genetic diversity and were not related to the geographical area. Notably, most strains (76.07%, 89/117) belonged to Mycobacterium paraintracellulare (MP) and Mycobacterium indicus pranii (MIP) in the genome, and we named them MP-MIP strains. These MP-MIP strains may be regarded as a causative agent of chronic lung disease. Furthermore, our data demonstrated that clarithromycin, amikacin, and rifabutin showed strong antimicrobial activity against both M. intracellulare and MP-MIP strains in vitro. Our findings also showed that there was no clear correlation between the rrs, rrl, and DNA gyrase genes (gyrA and gyrB) and the aminoglycosides, macrolides, and moxifloxacin resistance, respectively. In conclusion, this study highlights the high diversity of M. intracellulare in the clinical setting and suggests paying great attention to the lung disease caused by MP-MIP.
摘要：
胞内分枝杆菌是非结核性分枝杆菌肺病的最常见原因,在全球范围内迅速流行。在这项研究中,我们对中国117株临床胞内分枝杆菌进行了比较基因组分析和抗菌药物敏感性特征分析。系统发育分析表明，临床胞内分枝杆菌菌株具有较高的遗传多样性，与地理区域无关。值得注意的是,大多数菌株(76.07%，89/117）在基因组中属于副细胞分枝杆菌（MP）和印度分枝杆菌（MIP），我们将它们命名为MP-MIP菌株。这些MP-MIP菌株可被视为慢性肺病的病原体。此外，我们的数据表明克拉霉素，阿米卡星，利福布汀在体外对胞内分枝杆菌和MP-MIP菌株均显示出强的抗菌活性。我们的发现还表明rs之间没有明显的相关性，rrl,和DNA促旋酶基因（gyrA和gyrB）和氨基糖苷，大环内酯类,和莫西沙星耐药性，分别。总之,这项研究强调了临床环境中细胞内分枝杆菌的高度多样性，并建议高度重视由MP-MIP引起的肺部疾病。
