Abstract：
Advances in \"omics\" technology have made it possible to study a wide range of cellular phenomena at the single-cell level. Recently, we developed single-cell DNA replication sequencing (scRepli-seq) that measures replication timing (RT) by copy number differences between replicated and unreplicated genomic DNA in replicating single mammalian cells. This method has been used to reveal previously unrecognized static and dynamic natures of several hundred kilobases to a few megabases-scale chromosomal units called RT domains. Because RT domains are highly correlated to A/B compartments detected by Hi-C, scRepli-seq data can be used to predict the 3D organization of the genome in the nuclear space. scRepli-seq, which essentially measures the copy number, can also be applied to study genome instability.
摘要：
“组学”技术的进步使得在单细胞水平上研究广泛的细胞现象成为可能。最近,我们开发了单细胞DNA复制测序(scRepli-seq),通过复制的单个哺乳动物细胞中复制和未复制基因组DNA之间的拷贝数差异来测量复制时间(RT).此方法已用于揭示先前未识别的数百千碱基到称为RT域的几兆碱基规模的染色体单位的静态和动态性质。因为RT结构域与Hi-C检测到的A/B区室高度相关，scRepli-seq数据可用于预测核空间中基因组的3D组织。scRepli-seq,本质上是测量拷贝数的，也可用于研究基因组不稳定性。
