Abstract：
The quantification of parent molecules of pyrethroids tetramethrin and resmethrin in human specimens by a mass spectrometry (MS) technique has not been reported yet. A woman in her 60s was found dead in a wasteland. At the scene, an empty beer can and a spray for insecticides containing tetramethrin and resmethrin were found. Therefore, the concentrations of tetramethrin and resmethrin in postmortem specimens and the methanol solution used for rinsing the inside of the beer can were determined using liquid chromatography (LC)-tandem mass spectrometry (MS/MS).
The quantification method by LC-MS/MS for intact parent molecules of tetramethrin and resmethrin in whole blood and urine has been devised and validated in this work. The method was applied to the quantification of tetramethrin and resmethrin in whole blood, urine and stomach contents obtained from a cadaver at autopsy.
The limits of detection of tetramethrin and resmethrin were 0.06 and 0.03 ng/mL; limits of quantification were 0.2 and 0.1 ng/mL in blood and urine, respectively. The concentrations of tetramethrin of the deceased were 11.1 ± 1.2 and 0.425 ± 0.017 ng/mL for stomach contents and urine, respectively; the concentration of resmethrin in stomach contents was 1.77 ± 0.18 ng/mL. The tetramethrin and resmethrin were unstable in blood and urine at room temperature; they should be kept at not higher than 4 ℃.
To our knowledge, this is the first report for quantification of unchanged tetramethrin and resmethrin in human specimens obtained in a fatal case.
The quantification method by LC-MS/MS for intact parent molecules of tetramethrin and resmethrin in whole blood and urine has been devised and validated in this work. The method was applied to the quantification of tetramethrin and resmethrin in whole blood, urine and stomach contents obtained from a cadaver at autopsy.
The limits of detection of tetramethrin and resmethrin were 0.06 and 0.03 ng/mL; limits of quantification were 0.2 and 0.1 ng/mL in blood and urine, respectively. The concentrations of tetramethrin of the deceased were 11.1 ± 1.2 and 0.425 ± 0.017 ng/mL for stomach contents and urine, respectively; the concentration of resmethrin in stomach contents was 1.77 ± 0.18 ng/mL. The tetramethrin and resmethrin were unstable in blood and urine at room temperature; they should be kept at not higher than 4 ℃.
To our knowledge, this is the first report for quantification of unchanged tetramethrin and resmethrin in human specimens obtained in a fatal case.
摘要：
尚未报道通过质谱（MS）技术对人体标本中拟除虫菊酯四胺菊酯和瑞氯菊酯的母体分子进行定量。一名60多岁的妇女被发现死在荒地上。在现场,发现了一个空啤酒罐和一种含有胺菊酯和氯菊酯的杀虫剂喷雾剂。因此,使用液相色谱（LC）-串联质谱法（MS/MS）测定了死后标本中的四胺菊酯和氯菊酯的浓度以及用于冲洗啤酒罐内部的甲醇溶液。
在这项工作中，已经设计并验证了通过LC-MS/MS对全血和尿液中氨菊酯和瑞氯菊酯的完整母体分子的定量方法。该方法用于全血中氰菊酯和瑞氯菊酯的定量。尸检时从尸体中获得的尿液和胃内容物。
胺菊酯和氯菊酯的检出限为0.06和0.03ng/mL；血液和尿液中的定量限分别为0.2和0.1ng/mL，分别。死者的胃内容物和尿液中四胺菊酯的浓度分别为11.1±1.2和0.425±0.017ng/mL，胃内容物中瑞氯菊酯的浓度分别为1.77±0.18ng/mL。氨菊酯和瑞氯菊酯在常温下在血液和尿液中不稳定；它们应保持在不高于4℃的温度下。
据我们所知,这是在一个致命病例中获得的人体样本中对未改变的胺菊酯和氯菊酯进行定量的第一份报告。
在这项工作中，已经设计并验证了通过LC-MS/MS对全血和尿液中氨菊酯和瑞氯菊酯的完整母体分子的定量方法。该方法用于全血中氰菊酯和瑞氯菊酯的定量。尸检时从尸体中获得的尿液和胃内容物。
胺菊酯和氯菊酯的检出限为0.06和0.03ng/mL；血液和尿液中的定量限分别为0.2和0.1ng/mL，分别。死者的胃内容物和尿液中四胺菊酯的浓度分别为11.1±1.2和0.425±0.017ng/mL，胃内容物中瑞氯菊酯的浓度分别为1.77±0.18ng/mL。氨菊酯和瑞氯菊酯在常温下在血液和尿液中不稳定；它们应保持在不高于4℃的温度下。
据我们所知,这是在一个致命病例中获得的人体样本中对未改变的胺菊酯和氯菊酯进行定量的第一份报告。
