PDF(Pubmed)
Abstract：
Epimedium pubescens is a species of the family Berberidaceae in the basal eudicot lineage, and a main plant source for the traditional Chinese medicine \"Herba Epimedii\". The current study achieved a chromosome-level genome assembly of E. pubescens with the genome size of 3.34 Gb, and the genome guided discovery of a key prenyltransferase (PT) in E. pubescens. Our comparative genomic analyses confirmed the absence of Whole Genome Triplication (WGT-γ) event shared in core eudicots and further revealed the occurrence of an ancient Whole Genome Duplication (WGD) event approximately between 66 and 81 Million Years Ago (MYA). In addition, whole genome search approach was successfully applied to identify 19 potential flavonoid PT genes and an important flavonoid PT (EpPT8) was proven to be an enzyme for the biosynthesis of medicinal compounds, icaritin and its derivatives in E. pubescens. Therefore, our results not only provide a good reference genome to conduct further molecular biological studies in Epimedium genus, but also give important clues for synthetic biology and industrial production of related prenylated flavonoids in future.
摘要：
淫羊藿是基础eudicot谱系中小檗科的一种，和中药“淫羊藿”的主要植物来源。当前的研究实现了基因组大小为3.34Gb的毛竹的染色体水平基因组组装，和基因组指导的关键异戊烯基转移酶（PT）的发现。我们的比较基因组分析证实了核心eudicots中不存在全基因组重复（WGT-γ）事件，并进一步揭示了大约在66至8100万年前（MYA）之间发生的古代全基因组重复（WGD）事件。此外,全基因组搜索方法被成功地应用于鉴定19个潜在的类黄酮PT基因和重要的类黄酮PT（EpPT8）被证明是用于药物化合物的生物合成的酶，淫羊藿苷及其衍生物。因此,我们的研究结果不仅为进一步开展淫羊藿属的分子生物学研究提供了良好的参考基因组,同时也为今后相关异戊烯黄酮的合成生物学和工业化生产提供了重要线索。
