Abstract：
Early blight (EB), caused by Alternaria solani, is one of the alarming diseases that restrict tomato production globally. Existing cultural practices and fungicide applications are not enough to control early blight diseases. Therefore, the study aimed to isolate, identify, and characterize an endophytic Streptomyces exhibiting the potential to control early blight in tomato and also promote plant growth.
From a Citrus jambhiri leaf, an endophytic Streptomyces sp. with antagonistic activity against Alternaria solani, Colletotrichum acutatum, Cladosporium herbarum, Alternaria brassicicola, Alternaria sp., Fusarium oxysporum and Fusarium sp. was isolated. It was identified as a Streptomyces sp. through 16S ribosomal DNA sequence analysis and designated as SP5. It also produced indole acetic acid which was confirmed by Salkowski reagent assay, TLC and HPLC analysis. Treatment of pathogen infected plants with Streptomyces sp. SP5 antagonists (culture cells/culture supernatant/solvent extract/ acetone precipitates) decreased the early blight disease incidence and significantly increased the various agronomic traits.
The present study concluded that Streptomyces sp. SP5 possessed antifungal activity against different fungal phytopathogens and had significant potential to control early blight disease and promote plant growth.
From a Citrus jambhiri leaf, an endophytic Streptomyces sp. with antagonistic activity against Alternaria solani, Colletotrichum acutatum, Cladosporium herbarum, Alternaria brassicicola, Alternaria sp., Fusarium oxysporum and Fusarium sp. was isolated. It was identified as a Streptomyces sp. through 16S ribosomal DNA sequence analysis and designated as SP5. It also produced indole acetic acid which was confirmed by Salkowski reagent assay, TLC and HPLC analysis. Treatment of pathogen infected plants with Streptomyces sp. SP5 antagonists (culture cells/culture supernatant/solvent extract/ acetone precipitates) decreased the early blight disease incidence and significantly increased the various agronomic traits.
The present study concluded that Streptomyces sp. SP5 possessed antifungal activity against different fungal phytopathogens and had significant potential to control early blight disease and promote plant growth.
摘要：
早疫病（EB），由SolaniAlternaria引起，是限制全球番茄生产的令人担忧的疾病之一。现有的文化实践和杀真菌剂应用不足以控制早期疫病。因此,这项研究旨在隔离，identify,并表征了具有控制番茄早疫病并促进植物生长的潜力的内生链霉菌。
从柑橘叶，内生链霉菌属。具有对抗Solani链格孢菌的活性，炭疽病,草根孢霉,油菜链块菌,Alternariasp.,尖孢镰刀菌和镰刀菌。被隔离。它被鉴定为链霉菌属。通过16S核糖体DNA序列分析并定名为SP5。它还产生吲哚乙酸，通过Salkowski试剂测定法证实，TLC和HPLC分析。用链霉菌处理病原体感染的植物。SP5拮抗剂（培养细胞/培养上清液/溶剂提取物/丙酮沉淀物）降低了早期疫病的发病率，并显着增加了各种农艺性状。
本研究得出结论，链霉菌属。SP5具有对不同真菌植物病原菌的抗真菌活性，具有控制早期疫病和促进植物生长的显着潜力。
从柑橘叶，内生链霉菌属。具有对抗Solani链格孢菌的活性，炭疽病,草根孢霉,油菜链块菌,Alternariasp.,尖孢镰刀菌和镰刀菌。被隔离。它被鉴定为链霉菌属。通过16S核糖体DNA序列分析并定名为SP5。它还产生吲哚乙酸，通过Salkowski试剂测定法证实，TLC和HPLC分析。用链霉菌处理病原体感染的植物。SP5拮抗剂（培养细胞/培养上清液/溶剂提取物/丙酮沉淀物）降低了早期疫病的发病率，并显着增加了各种农艺性状。
本研究得出结论，链霉菌属。SP5具有对不同真菌植物病原菌的抗真菌活性，具有控制早期疫病和促进植物生长的显着潜力。
