PDF(Pubmed)
Abstract：
The thermophilic spore-forming strain Geobacillus sp. CX412 was isolated from hot spring soil in Tengchong City, Yunnan Province, China. We sequenced the complete genome of Geobacillus sp. CX412 using PacBio SMRT Sequencing. Genome-scale phylogenetic analysis and average nucleotide identity (ANI) results indicated that Geobacillus sp. CX412 is a novel species in the genus Geobacillus. The metabolic potential of Geobacillus sp. CX412 based on COG, KEGG, and CAZymes analysis demonstrated that Geobacillus sp. CX412 was a highly adaptable strain with an unusually high number of 73 annotated transposons in the genome, which is relatively rare in Geobacillus. Compared with the near-derived strains, it was found that Geobacillus sp. CX412 has the unique β-lactam resistance and more active metabolism (more than 50.5-100.1%). Additionally, its genome encodes glycoside hydrolases and other genes related to lignocellulose breakdown, suggesting that Geobacillus sp. CX412 has a considerable biomass degradation potential. Thus, Geobacillus sp. CX412 is a new thermophilic bacterial species that add to the increasing repertoire of known lignocellulose degraders.
摘要：
嗜热孢子形成菌株地芽孢杆菌。CX412从腾冲市温泉土壤中分离出来，云南省,中国。我们对地芽孢杆菌的完整基因组进行了测序。CX412使用PacBioSMRT测序。基因组规模的系统发育分析和平均核苷酸同一性（ANI）结果表明，地芽孢杆菌。CX412是地芽孢杆菌属中的新物种。地芽孢杆菌的代谢潜力。基于COG的CX412，KEGG,和CAZymes分析表明，地芽孢杆菌。CX412是一个适应性很强的菌株，在基因组中具有异常高的73个注释转座子，这在地芽孢杆菌中相对罕见。与近衍生菌株相比，发现地芽孢杆菌。CX412具有独特的β-内酰胺抗性和更活跃的代谢(超过50.5-100.1%)。此外,它的基因组编码糖苷水解酶和其他与木质纤维素分解相关的基因，表明地芽孢杆菌。CX412具有相当大的生物质降解潜力。因此，地芽孢杆菌。CX412是一种新的嗜热细菌物种，它增加了已知的木质纤维素降解剂的数量。
