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Abstract：
The role of noncoding RNAs (ncRNAs) in plant resistance to abiotic stresses is increasingly being discovered. Drought stress is one of the most common stresses that affecting plant growth, and high intensity drought has a significant impact on the normal growth of plants. In this study, a high-throughput sequencing was performed on plant tissue samples of Phyllostachys aureosulcata f. spectabilis C. D. Chu et C. S. Chao by drought treatment for 0, 2, 4 and 6 days. The sequencing results were analysed bioinformatically. We detected 336,946 RNAs among all 12 samples, including 192,098 message RNAs (mRNAs), 142,761 long noncoding RNAs (lncRNAs), 1,670 circular RNAs (circRNAs), and 417 microRNAs (miRNAs). We detected 2,419 differentially expressed (DE) ncRNAs, including 213 DE circRNAs, 2,088 DE lncRNAs and 118 DE miRNAs. Then, we used Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) to functionally predict DE ncRNAs. The results showed that most DE ncRNAs are involved in the response to drought stress, mainly in biochemical reactions involved in some metabolites, as well as in organelle activities. In addition, we validated two random circRNAs and demonstrated their circularity. We also found a stable internal reference gene available for Phyllostachys aureosulcata f. spectabilis and validated the accuracy of this experiment by quantitative real-time polymerase chain reaction (qRT-PCR).
摘要：
非编码RNA（ncRNA）在植物对非生物胁迫的抗性中的作用日益被发现。干旱胁迫是影响植物生长的最常见胁迫之一。高强度干旱对植物的正常生长有显著影响。在这项研究中,通过干旱处理0、2、4和6天，对毛竹的植物组织样品进行了高通量测序。对测序结果进行生物信息学分析。我们在所有12个样本中检测到336,946个RNA，包括192,098个信息RNA（mRNA），142,761长非编码RNA（lncRNAs），1,670个环状RNA（circRNAs），和417个微小RNA(miRNA)。我们检测到2,419个差异表达（DE）ncRNAs，包括213个DEcircRNAs，2,088DElncRNAs和118DEmiRNAs。然后，我们使用基因本体论（GO）和京都基因和基因组百科全书（KEGG）功能预测DEncRNAs。结果表明,大部分DEncRNAs参与了干旱胁迫的响应,主要涉及一些代谢物的生化反应，以及在细胞器活动中。此外,我们验证了两个随机circRNAs并证明了它们的圆度。我们还发现了一个稳定的内参基因可用于Phyllostachysaurealossulcataf。pectabilis，并通过定量实时聚合酶链反应（qRT-PCR）验证了该实验的准确性。
