PDF(Pubmed)
Abstract：
The intestinal microbiome is essential to human health and homeostasis, and is implicated in the pathophysiology of disease, including congenital heart disease and cardiac surgery. Improving the microbiome and reducing inflammatory metabolites may reduce systemic inflammation following cardiac surgery with cardiopulmonary bypass (CPB) to expedite recovery post-operatively. Limited research exists in this area and identifying animal models that can replicate changes in the human intestinal microbiome after CPB is necessary. We used a piglet model of CPB with two groups, CPB (n=5) and a control group with mechanical ventilation (n=7), to evaluate changes to the microbiome, intestinal barrier dysfunction and intestinal metabolites with inflammation after CPB. We identified significant changes to the microbiome, barrier dysfunction, intestinal short-chain fatty acids and eicosanoids, and elevated cytokines in the CPB/deep hypothermic circulatory arrest group compared to the control group at just 4 h after intervention. This piglet model of CPB replicates known human changes to intestinal flora and metabolite profiles, and can be used to evaluate gut interventions aimed at reducing downstream inflammation after cardiac surgery with CPB.
摘要：
肠道微生物对人类健康和体内平衡至关重要，并且与疾病的病理生理学有关。包括先天性心脏病和心脏手术.改善微生物组和减少炎性代谢物可以减少心脏手术后的全身炎症与体外循环（CPB），以加快术后恢复。该领域的研究有限，需要确定可以复制CPB后人体肠道微生物组变化的动物模型。我们采用仔猪CPB模型，分为2组，CPB（n=5）和对照组机械通气（n=7）以评估微生物组的变化，肠屏障功能障碍，和肠道代谢产物与CPB后的炎症。我们发现了微生物组的重大变化，屏障功能障碍,肠道短链脂肪酸和类二十烷酸，与对照组相比，CPB/DHCA组的细胞因子在干预后仅4小时升高。这种CPB的仔猪模型复制了肠道菌群和代谢物分布的已知人类变化，可用于评估旨在减少CPB心脏手术后下游炎症的肠道干预措施。
