PDF(Pubmed)
Abstract：
Insect-specific virus (ISV) is one of the most promising agents for the biological control of insects, which is abundantly distributed in hematophagous insects. However, few ISVs have been reported in Riptortus pedestris (Fabricius), one of the major pests threatening soybeans and causing great losses in yield and quality. In this work, field Riptortus pedestris was collected from six soybean-producing regions in China, and their virome was analyzed with the metatranscriptomic approach. Altogether, seven new insect RNA viruses were identified, three of which had complete RNA-dependent RNA polymerase (RdRp) and nearly full-length genome sequences, which were named Riptortus pedestris alphadrosrha-like virus 1 (RpALv1), Riptortus pedestris alphadrosrha-like virus 2 (RpALv2) and Riptortus pedestris almendra-like virus (RiALv). The three identified novel ISVs belonged to the family Rhabdoviridae, and phylogenetic tree analysis indicated that they were clustered into new distinct clades. Interestingly, the analysis of virus-derived small-interfering RNAs (vsiRNAs) indicated that only RiALv-derived siRNAs exhibited 22 nt length preference, whereas no clear 21 or 22 nt peaks were observed for RpALv1 and RpALv2, suggesting the complexity of siRNA-based antiviral immunity in R. pedestris. In conclusion, this study contributes to a better understanding of the microenvironment in R. pedestris and provides viral information for the development of potential soybean insect-specific biocontrol agents.
摘要：
昆虫特异性病毒(ISV)是最有前途的生物防治昆虫的药剂之一。大量分布在吸血昆虫中。然而,在Riptortuspedestris(Fabricius)中报道了很少的ISV，威胁大豆的主要害虫之一，造成产量和品质的巨大损失。在这项工作中,从中国六个大豆产区采集了稻田Riptortuspedestris，和他们的病毒学分析与metatranscriptomy方法。总之,发现了7种新的昆虫RNA病毒,其中三个具有完整的RNA依赖性RNA聚合酶（RdRp）和几乎全长的基因组序列，被命名为Riptortuspedestrisalphadrosrha样病毒1（RpALv1），鱼腥草样病毒2型（RpALv2）和鱼腥草样病毒（RiALv）。三个鉴定的新型ISV属于弹状病毒科，系统发育树分析表明，它们聚集成新的不同的进化枝。有趣的是,对病毒来源的小干扰RNA（vsiRNAs）的分析表明，只有RiALv来源的siRNA表现出22nt长度偏好，而在RpALv1和RpALv2中未观察到明确的21或22nt峰，这表明基于siRNA的抗病毒免疫的复杂性。总之,这项研究有助于更好地了解R.pedestris的微环境，并为开发潜在的大豆昆虫特异性生物防治剂提供病毒信息。
