Abstract：
Simple staining of cells is a widely used method in basic medical diagnostics, education, and research laboratories. The stains are low-cost, but the extensive consumption results in excessive toxic waste generation. Thus, to decrease the amount of toxic waste resulting from the cell staining procedure is a need. In this study, we developed a magnetically driven and compartmentalized passive microfluidic chip to perform simple staining of human eukaryotic cells, K562 cells, and lymphocyte cells derived from patients. We demonstrated simple staining on cells with trypan blue, methylene blue, crystal violet, and safranin for high, medium, and low cell densities. The stained cells were imaged using a bright field optical microscope and a cell phone to count cells on the focal plane. The staining improved the color signal of the cell by 25-135-pixel intensity changes for the microscopic images. The validity of the protocol was determined using Jurkat and MDA-MB-231 cell lines as negative controls. In order to demonstrate the practicality of the system, lymphocyte cells derived from human blood samples were stained with trypan blue. The color intensity changes in the first and last compartments were analyzed to evaluate the performance of the chip. The developed method is ultra-low cost, significantly reduces the waste generated, and can be integrated with mobile imaging devices in terms of portability. By combining microfabrication technology with cell staining, this study reported a novel contribution to the field of microfluidic biosensors. In the future, we expect to demonstrate the detection of pathogens using this method.
摘要：
简单的细胞染色是一种广泛用于基础医学诊断的方法，教育,和研究实验室。污渍成本低，但是大量消费导致过量的有毒废物产生。因此，需要减少细胞染色程序产生的有毒废物的量。在这项研究中,我们开发了一种磁驱动和分隔的无源微流控芯片来对人类真核细胞进行简单的染色，K562细胞,和来自患者的淋巴细胞。我们展示了用锥虫蓝对细胞的简单染色，亚甲蓝,结晶紫,和高的Safranin，中等,和低细胞密度。使用明场光学显微镜和手机对染色的细胞进行成像以在焦平面上计数细胞。染色使细胞的颜色信号改善了显微图像的25-135像素的强度变化。使用Jurkat和MDA-MB-231细胞系作为阴性对照确定方案的有效性。为了证明系统的实用性,来源于人血样的淋巴细胞用台盼蓝染色。分析第一个和最后一个隔室中的颜色强度变化以评估芯片的性能。所开发的方法是超低成本的，大大减少产生的废物，并且可以在便携性方面与移动成像设备集成。通过将微细加工技术与细胞染色相结合，这项研究报告了对微流控生物传感器领域的新贡献。在未来,我们希望证明使用这种方法检测病原体。
