Abstract：
Primary human dental pulp cell (HDPC) cultures contain dental pulp stem cell (DPSC) populations. DPSCs are multipotent mesenchymal cells residing inside the dental pulp where they can contribute to the regenerative potential of this and other tissues throughout the body. These cells are promising tools for cell-based therapies, including regenerative endodontic procedures. HDPCs can be readily isolated and expanded from extracted teeth either by the dental tissue explant method or enzymatic digestion method. This chapter describes the explant method, whereby cells outgrow from dissected pulp tissue, to generate HDPC cultures. We also provide protocols for HDPC passaging, cryopreservation, and basic immunocytochemical characterization.
摘要：
原代人牙髓细胞(HDPC)培养物含有牙髓干细胞(DPSC)群体。DPSC是存在于牙髓内部的多能间充质细胞,其中它们可以有助于整个身体的该组织和其他组织的再生潜能。这些细胞是细胞疗法的有前途的工具，包括再生牙髓手术。HDPC可以通过牙齿组织外植体方法或酶消化方法容易地从提取的牙齿中分离和扩增。本章介绍了外植体的方法，细胞从解剖的牙髓组织中生长出来，生成HDPC文化。我们还提供HDPC传送协议，冷冻保存,和基本的免疫细胞化学表征。
