Abstract：
The T-shaped radial spoke (RS) is a protein complex attached to the A microtubule of the outer doublet microtubules, and it extends toward the central pair (CP). It modulates the beat frequency, amplitude, and waveform of the flagella and cilia by serving as a mechanochemical signal transducer between the CP and dyneins. In humans, RS defects cause primary ciliary dyskinesia, but the structural components of triple RSs (RS1, RS2, and RS3) in mammals remain to be elucidated in detail. Here, we introduce a mouse model that lacked the entire RS1 in sperm flagella, due to the deletion of Iqub, while the tracheal cilia possessed intact triple RSs. Furthermore, the absence of IQUB only resulted in male infertility, owing to the sperm motility defect. Based on the mouse model, the RS1 compositions are identified in sperm flagella. In summary, this study elucidates the RS1 components and function in mammalian flagella.
摘要：
T形径向辐条（RS）是一种蛋白质复合物，附着在外部双联微管的A微管上，并且它向中心对(CP)延伸。它调制拍频，振幅,以及鞭毛和纤毛的波形，作为CP和动力蛋白之间的机械化学信号换能器。在人类中,RS缺陷导致原发性纤毛运动障碍，但是哺乳动物中三重RS（RS1，RS2和RS3）的结构成分仍有待详细阐明。这里,我们介绍了一个精子鞭毛中缺乏整个RS1的小鼠模型，由于Iqub的删除，气管纤毛具有完整的三重RS。此外，缺乏IQUB只会导致男性不育，由于精子运动缺陷。基于小鼠模型,在精子鞭毛中鉴定RS1组合物。总之,这项研究阐明了哺乳动物鞭毛中RS1的成分和功能。
