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Abstract：
BACKGROUND: The early diagnosis of sepsis is beneficial to put forward a reasonable clinical treatment plan as soon as possible. This study was to explore the expression of Tripartite Motif 7 (TRIM7) in peripheral blood mononuclear cells (PBMCs) of patients with sepsis and its diagnostic value.
METHODS: This is a cross-sectional study. A total of 69 patients with infectious diseases were enrolled in the emergency room. They were divided into the sepsis group (34 cases) and the non-sepsis infection group (35 cases). There were 25 healthy subjects who were selected as the control group. The expression of TRIM7 in PBMCs was observed by immunofluorescence staining. The correlation between the expression of TRIM7 mRNA and acute physiology and chronic health evaluation II (APACHE II) score, sequential organ failure assessment (SOFA) score, white blood cell (WBC), C-reactive protein (CRP), procalcitonin (PCT), tumor necrosis factor (TNF)-α and interleukin (IL)-6 was discussed. The receiver operating characteristic (ROC) curve was utilized for evaluating the value of TRIM7 expression for the early diagnosis of sepsis.
RESULTS: The fluorescence intensity representing the expression level of TRIM7 in PBMCs of patients in the sepsis group was the lowest among three groups. The TRIM7 mRNA expression in PBMCs of the sepsis group was greatly decreased in comparison with that of the non-sepsis infection group and control group (P < 0.05). Spearman correlation analysis indicated that TRIM7 mRNA expression was negatively correlated with APACHE II score, SOFA score, WBC, CRP, PCT, TNF-α and IL-6. ROC curve analysis revealed that the area under curve (AUC) of TRIM7 mRNA expression in PBMCs for the diagnosis of sepsis was 0.798, with a 95% confidence interval of 0.691- 0.905, a sensitivity of 73.5%, and a specificity of 77.1%.
CONCLUSIONS: The expression of TRIM7 in PBMCs of patients with sepsis is significantly down-regulated, which has certain clinical value for early diagnosis of sepsis.
METHODS: This is a cross-sectional study. A total of 69 patients with infectious diseases were enrolled in the emergency room. They were divided into the sepsis group (34 cases) and the non-sepsis infection group (35 cases). There were 25 healthy subjects who were selected as the control group. The expression of TRIM7 in PBMCs was observed by immunofluorescence staining. The correlation between the expression of TRIM7 mRNA and acute physiology and chronic health evaluation II (APACHE II) score, sequential organ failure assessment (SOFA) score, white blood cell (WBC), C-reactive protein (CRP), procalcitonin (PCT), tumor necrosis factor (TNF)-α and interleukin (IL)-6 was discussed. The receiver operating characteristic (ROC) curve was utilized for evaluating the value of TRIM7 expression for the early diagnosis of sepsis.
RESULTS: The fluorescence intensity representing the expression level of TRIM7 in PBMCs of patients in the sepsis group was the lowest among three groups. The TRIM7 mRNA expression in PBMCs of the sepsis group was greatly decreased in comparison with that of the non-sepsis infection group and control group (P < 0.05). Spearman correlation analysis indicated that TRIM7 mRNA expression was negatively correlated with APACHE II score, SOFA score, WBC, CRP, PCT, TNF-α and IL-6. ROC curve analysis revealed that the area under curve (AUC) of TRIM7 mRNA expression in PBMCs for the diagnosis of sepsis was 0.798, with a 95% confidence interval of 0.691- 0.905, a sensitivity of 73.5%, and a specificity of 77.1%.
CONCLUSIONS: The expression of TRIM7 in PBMCs of patients with sepsis is significantly down-regulated, which has certain clinical value for early diagnosis of sepsis.
摘要：
背景：脓毒症的早期诊断有利于尽早提出合理的临床治疗方案。本研究旨在探讨三方基序7(TRIM7)在脓毒症患者外周血单个核细胞(PBMCs)中的表达及其诊断价值。
方法：这是一项横断面研究。共有69例感染性疾病患者进入急诊室。将其分为脓毒症组(34例)和非脓毒症感染组(35例)。选择25例健康体检者作为对照组。免疫荧光染色观察TRIM7在PBMCs中的表达。TRIM7mRNA表达与急性生理和慢性健康评价Ⅱ(APACHEⅡ)评分的相关性,序贯器官衰竭评估（SOFA）评分，白细胞（WBC），C反应蛋白(CRP),降钙素原(PCT),讨论了肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6。利用受试者工作特征(ROC)曲线评价TRIM7表达对脓毒症早期诊断的价值。
结果：代表脓毒症组患者PBMC中TRIM7表达水平的荧光强度在三组中最低。脓毒症组PBMC中TRIM7mRNA的表达水平显著低于非脓毒症感染组和对照组(P<0.05)。Spearman相关分析显示TRIM7mRNA表达与APACHEⅡ评分呈负相关,SOFA得分,WBC,CRP,PCT,TNF-α和IL-6。ROC曲线分析显示PBMC中TRIM7mRNA表达对脓毒症诊断的曲线下面积(AUC)为0.798,95%置信区间为0.691-0.905,敏感性为73.5%。特异性为77.1%。
结论：脓毒症患者PBMC中TRIM7的表达明显下调，对脓毒症的早期诊断具有一定的临床价值。
方法：这是一项横断面研究。共有69例感染性疾病患者进入急诊室。将其分为脓毒症组(34例)和非脓毒症感染组(35例)。选择25例健康体检者作为对照组。免疫荧光染色观察TRIM7在PBMCs中的表达。TRIM7mRNA表达与急性生理和慢性健康评价Ⅱ(APACHEⅡ)评分的相关性,序贯器官衰竭评估（SOFA）评分，白细胞（WBC），C反应蛋白(CRP),降钙素原(PCT),讨论了肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6。利用受试者工作特征(ROC)曲线评价TRIM7表达对脓毒症早期诊断的价值。
结果：代表脓毒症组患者PBMC中TRIM7表达水平的荧光强度在三组中最低。脓毒症组PBMC中TRIM7mRNA的表达水平显著低于非脓毒症感染组和对照组(P<0.05)。Spearman相关分析显示TRIM7mRNA表达与APACHEⅡ评分呈负相关,SOFA得分,WBC,CRP,PCT,TNF-α和IL-6。ROC曲线分析显示PBMC中TRIM7mRNA表达对脓毒症诊断的曲线下面积(AUC)为0.798,95%置信区间为0.691-0.905,敏感性为73.5%。特异性为77.1%。
结论：脓毒症患者PBMC中TRIM7的表达明显下调，对脓毒症的早期诊断具有一定的临床价值。
