Abstract：
Reef-building corals play an important role in the marine ecosystem, and analyzing their proteomes from a structural perspective will exert positive effects on exploring their biology. Here we integrated mass spectrometry with newly published ColabFold to obtain digital structural proteomes of dominant reef-building corals.
Of the 8,382 homologous proteins in Acropora muricata, Montipora foliosa, and Pocillopora verrucosa identified, 8,166 received predicted structures after about 4,060 GPU hours of computation. The resulting dataset covers 83.6% of residues with a confident prediction, while 25.9% have very high confidence.
Our work provides insight-worthy predictions for coral research, confirms the reliability of ColabFold in practice, and is expected to be a reference case in the impending high-throughput era of structural proteomics.
Of the 8,382 homologous proteins in Acropora muricata, Montipora foliosa, and Pocillopora verrucosa identified, 8,166 received predicted structures after about 4,060 GPU hours of computation. The resulting dataset covers 83.6% of residues with a confident prediction, while 25.9% have very high confidence.
Our work provides insight-worthy predictions for coral research, confirms the reliability of ColabFold in practice, and is expected to be a reference case in the impending high-throughput era of structural proteomics.
摘要：
造礁珊瑚在海洋生态系统中发挥着重要作用,从结构角度分析它们的蛋白质组将对探索它们的生物学产生积极影响。在这里，我们将质谱与新发表的ColabFold集成在一起，以获得主要的造礁珊瑚的数字结构蛋白质组。
在Acroporamuricata中的8,382个同源蛋白中，Montiporafoliosa,并鉴定出疣状孢子虫，8,166在大约4,060个GPU小时的计算后收到了预测的结构。得到的数据集覆盖了83.6%的残基，具有自信的预测，而25.9%的人有很高的信心。
我们的工作为珊瑚研究提供了有洞察力的预测，证实了ColabFold在实践中的可靠性，并有望成为结构蛋白质组学即将到来的高通量时代的参考案例。
在Acroporamuricata中的8,382个同源蛋白中，Montiporafoliosa,并鉴定出疣状孢子虫，8,166在大约4,060个GPU小时的计算后收到了预测的结构。得到的数据集覆盖了83.6%的残基，具有自信的预测，而25.9%的人有很高的信心。
我们的工作为珊瑚研究提供了有洞察力的预测，证实了ColabFold在实践中的可靠性，并有望成为结构蛋白质组学即将到来的高通量时代的参考案例。
