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Abstract：
OBJECTIVE: This study aims to investigate the cytokines profiling in the aqueous humor of patients with polypoidal choroidal vasculopathy (PCV) before and after intravitreal ranibizumab injection (IVR).
METHODS: 14 patients clinically diagnosed with PCV and 15 cataract patients of similar age and gender (control group) were included. Throughout the cataract surgery and IVR, aqueous humor samples were collected from the PCV and control groups.
RESULTS: The levels of macrophage inflammatory protein 1β (MIP-1β) and normal T cell expressed and secreted (RANTES) in PCV patients were significantly lower than control subjects (P=0.045 and P=0.004, respectively). The concentration of vascular endothelial growth factor-A (VEGF-A) was significantly higher than the control group (P=0.003). The level of MIP-1β was greatly increased in PCV patients compared to prior to IVR (P=0.001). After IVR, the level of VEGF-A in PCV patients were considerably lower compared to before IVR (P=0.001). There was no link between the expression of several cytokines (MCP-1, MIP-1, Eotaxin, G-CSF, IL-8, IL-6, IL-5, IP-10 and IFN-γ) in the aqueous humor of PCV patients before and after intravitreal ranibizumab injection (IVR). The association between IL-5 expression and central macular thickness (CMT) was discovered before IVR (P=0.02), however, the correlation between several cytokines (MCP-1, MIP-1, Eotaxin, G-CSF, IL-8, IL-6, IL-5, IP-10 and IFN-γ) was discovered in PCV patients after IVR.
CONCLUSIONS: Based on our findings, we discovered that the production of neovascularization in PCV patients is driven by both angiogenic and inflammatory factors, with a correlation seen between several cytokines.
METHODS: 14 patients clinically diagnosed with PCV and 15 cataract patients of similar age and gender (control group) were included. Throughout the cataract surgery and IVR, aqueous humor samples were collected from the PCV and control groups.
RESULTS: The levels of macrophage inflammatory protein 1β (MIP-1β) and normal T cell expressed and secreted (RANTES) in PCV patients were significantly lower than control subjects (P=0.045 and P=0.004, respectively). The concentration of vascular endothelial growth factor-A (VEGF-A) was significantly higher than the control group (P=0.003). The level of MIP-1β was greatly increased in PCV patients compared to prior to IVR (P=0.001). After IVR, the level of VEGF-A in PCV patients were considerably lower compared to before IVR (P=0.001). There was no link between the expression of several cytokines (MCP-1, MIP-1, Eotaxin, G-CSF, IL-8, IL-6, IL-5, IP-10 and IFN-γ) in the aqueous humor of PCV patients before and after intravitreal ranibizumab injection (IVR). The association between IL-5 expression and central macular thickness (CMT) was discovered before IVR (P=0.02), however, the correlation between several cytokines (MCP-1, MIP-1, Eotaxin, G-CSF, IL-8, IL-6, IL-5, IP-10 and IFN-γ) was discovered in PCV patients after IVR.
CONCLUSIONS: Based on our findings, we discovered that the production of neovascularization in PCV patients is driven by both angiogenic and inflammatory factors, with a correlation seen between several cytokines.
摘要：
目的：本研究旨在研究玻璃体内注射雷珠单抗（IVR）前后息肉状脉络膜血管病变（PCV）患者房水中的细胞因子谱。
方法：包括14例临床诊断为PCV的患者和15例年龄和性别相似的白内障患者（对照组）。在整个白内障手术和IVR中，收集PCV组和对照组的房水样本.
结果：PCV患者的巨噬细胞炎性蛋白1β（MIP-1β）和正常T细胞表达和分泌（RANTES）水平明显低于对照组（分别为P=0.045和P=0.004）。血管内皮生长因子-A(VEGF-A)浓度明显高于对照组(P=0.003)。与IVR之前相比，PCV患者的MIP-1β水平大大增加（P=0.001）。在IVR之后,PCV患者的VEGF-A水平明显低于IVR前(P=0.001).几种细胞因子的表达之间没有联系(MCP-1，MIP-1，Eotaxin，G-CSF,在玻璃体内注射雷珠单抗（IVR）之前和之后，PCV患者的房水中的IL-8，IL-6，IL-5，IP-10和IFN-γ）。IVR前发现IL-5表达与黄斑中心厚度(CMT)的相关性(P=0.02),然而,几种细胞因子(MCP-1，MIP-1，Eotaxin，G-CSF,在IVR后的PCV患者中发现了IL-8，IL-6，IL-5，IP-10和IFN-γ）。
结论：根据我们的发现，我们发现PCV患者新生血管的产生是由血管生成和炎症因子共同驱动的,与几种细胞因子之间的相关性。
方法：包括14例临床诊断为PCV的患者和15例年龄和性别相似的白内障患者（对照组）。在整个白内障手术和IVR中，收集PCV组和对照组的房水样本.
结果：PCV患者的巨噬细胞炎性蛋白1β（MIP-1β）和正常T细胞表达和分泌（RANTES）水平明显低于对照组（分别为P=0.045和P=0.004）。血管内皮生长因子-A(VEGF-A)浓度明显高于对照组(P=0.003)。与IVR之前相比，PCV患者的MIP-1β水平大大增加（P=0.001）。在IVR之后,PCV患者的VEGF-A水平明显低于IVR前(P=0.001).几种细胞因子的表达之间没有联系(MCP-1，MIP-1，Eotaxin，G-CSF,在玻璃体内注射雷珠单抗（IVR）之前和之后，PCV患者的房水中的IL-8，IL-6，IL-5，IP-10和IFN-γ）。IVR前发现IL-5表达与黄斑中心厚度(CMT)的相关性(P=0.02),然而,几种细胞因子(MCP-1，MIP-1，Eotaxin，G-CSF,在IVR后的PCV患者中发现了IL-8，IL-6，IL-5，IP-10和IFN-γ）。
结论：根据我们的发现，我们发现PCV患者新生血管的产生是由血管生成和炎症因子共同驱动的,与几种细胞因子之间的相关性。
