Abstract：
BACKGROUND: Feeding by pests is one of the most important reasons for reductions in agricultural crop yield. This study aimed to reveal how juvenile hormone (JH) participates in larval feeding regulation of the Asian corn borer Ostrinia furnacalis.
RESULTS: Larvae of O. furnacalis exhibit a daily circadian feeding rhythm, with a peak at ZT18 and a trough at ZT6 under both photoperiod (LD) and constant dark (DD) conditions, which may be eliminated by application of fenoxycarb, a JH active analogue. JH negatively regulates larval feeding as a downstream factor of neuropeptide F (NPF), in which knocking down JH increases larval feeding amount along with body weight and length. The production of JH in the brain-corpora cardiaca-corpora allata (brain-CC-CA) is regulated by brain NPF rather than gut NPF, which was demonstrated in Drosophila larvae through GAL4/UAS genetic analysis. In addition, feeding regulation of JH is closely related to energy homeostasis in the fat body by inhibiting energy storage and promoting degradation. The JH analogue fenoxycarb is an effective pesticide against O. furnacalis, controlling feeding and metabolism.
CONCLUSIONS: The brain NPF system regulates JH, with functions in food consumption, feeding rhythms, energy homeostasis and body size. This study provides an important basis for understanding the feeding mechanism and potential pest control of O. furnacalis. © 2022 Society of Chemical Industry.
RESULTS: Larvae of O. furnacalis exhibit a daily circadian feeding rhythm, with a peak at ZT18 and a trough at ZT6 under both photoperiod (LD) and constant dark (DD) conditions, which may be eliminated by application of fenoxycarb, a JH active analogue. JH negatively regulates larval feeding as a downstream factor of neuropeptide F (NPF), in which knocking down JH increases larval feeding amount along with body weight and length. The production of JH in the brain-corpora cardiaca-corpora allata (brain-CC-CA) is regulated by brain NPF rather than gut NPF, which was demonstrated in Drosophila larvae through GAL4/UAS genetic analysis. In addition, feeding regulation of JH is closely related to energy homeostasis in the fat body by inhibiting energy storage and promoting degradation. The JH analogue fenoxycarb is an effective pesticide against O. furnacalis, controlling feeding and metabolism.
CONCLUSIONS: The brain NPF system regulates JH, with functions in food consumption, feeding rhythms, energy homeostasis and body size. This study provides an important basis for understanding the feeding mechanism and potential pest control of O. furnacalis. © 2022 Society of Chemical Industry.
摘要：
背景：害虫的饲养是农作物产量下降的最重要原因之一。这项研究旨在揭示保幼激素（JH）如何参与亚洲玉米boreOstriniafuracalis的幼虫摄食调节。
结果：火牛幼虫表现出每日昼夜节律，在光周期（LD）和恒定暗（DD）条件下，在ZT18处具有峰，在ZT6处具有谷，可以通过应用非诺昔卡来消除，aJH活性类似物。JH负调节幼虫摄食作为神经肽F（NPF）的下游因子，击倒JH会增加幼虫的摄取量以及体重和长度。JH在大脑-身体-身体-身体（brain-CC-CA）中的产生是由大脑NPF而不是肠道NPF调节的，通过GAL4/UAS遗传分析在果蝇幼虫中证明了这一点。此外,JH的摄食调节通过抑制能量储存和促进降解与脂肪体内的能量稳态密切相关。JH类似物fenoxycarb是一种有效的农药，控制进食和新陈代谢。
结论：大脑NPF系统调节JH，具有食物消费功能，喂食节奏，能量稳态和体型。本研究为了解火牛的摄食机理和潜在害虫防治提供了重要依据。©2022化学工业学会。
结果：火牛幼虫表现出每日昼夜节律，在光周期（LD）和恒定暗（DD）条件下，在ZT18处具有峰，在ZT6处具有谷，可以通过应用非诺昔卡来消除，aJH活性类似物。JH负调节幼虫摄食作为神经肽F（NPF）的下游因子，击倒JH会增加幼虫的摄取量以及体重和长度。JH在大脑-身体-身体-身体（brain-CC-CA）中的产生是由大脑NPF而不是肠道NPF调节的，通过GAL4/UAS遗传分析在果蝇幼虫中证明了这一点。此外,JH的摄食调节通过抑制能量储存和促进降解与脂肪体内的能量稳态密切相关。JH类似物fenoxycarb是一种有效的农药，控制进食和新陈代谢。
结论：大脑NPF系统调节JH，具有食物消费功能，喂食节奏，能量稳态和体型。本研究为了解火牛的摄食机理和潜在害虫防治提供了重要依据。©2022化学工业学会。
