Abstract：
Approximately half a million vasectomies are performed every year in the USA. There is a paucity of literature on the impact of male sterilization on the semen microbiome and whether it prompts microbiota dysbiosis.
To investigate if vasectomy induces changes in the seminal microbiome via comparison of semen samples from men before and after vasectomy, and if the seminal microbiome profiles for vasectomized men follow a particular pattern with respect to diversity and abundance.
From July 2021 to February 2022, we prospectively collected and analyzed semen samples from 58 men at one outpatient clinic. Eighteen men provided a semen sample before and 3 mo after vasectomy. We also collected semen samples from 22 fertile nonvasectomized men and from a further 18 vasectomized men at 3 mo after vasectomy.
Semen microbiome α-diversity, beta-diversity, and relative abundance were compared initially between paired and then between unpaired vasectomized and nonvasectomized samples. Analysis of variance (ANOVA), permutational multivariate ANOVA, and analysis of the composition of microbiomes with bias correction were used to assess differences.
In both paired and unpaired sets of samples, a decreasing trend for α-diversity in semen after vasectomy was observed. Shannon diversity, the relative abundance of species with an abundance >2%, and composition were not significantly changed. Sphingomonas, Brevundimonas, and Paracoccus abundance decreased after vasectomy, while Corynebacterium abundance increased. The results may be limited by the sample size and lack of demographic heterogeneity.
Vasectomy is followed by a decrease in α-diversity and changes in the relative abundance of bacterial species in the semen microbiome. Further investigation is necessary to understand the clinical significance of these changes after vasectomy.
We evaluated changes in the bacteria species in semen after vasectomy. We found that vasectomy decreased the richness and evenness of bacteria species in semen, but the overall bacterial community remained similar. Further studies are needed to assess the implications of changes in semen bacteria after vasectomy.
To investigate if vasectomy induces changes in the seminal microbiome via comparison of semen samples from men before and after vasectomy, and if the seminal microbiome profiles for vasectomized men follow a particular pattern with respect to diversity and abundance.
From July 2021 to February 2022, we prospectively collected and analyzed semen samples from 58 men at one outpatient clinic. Eighteen men provided a semen sample before and 3 mo after vasectomy. We also collected semen samples from 22 fertile nonvasectomized men and from a further 18 vasectomized men at 3 mo after vasectomy.
Semen microbiome α-diversity, beta-diversity, and relative abundance were compared initially between paired and then between unpaired vasectomized and nonvasectomized samples. Analysis of variance (ANOVA), permutational multivariate ANOVA, and analysis of the composition of microbiomes with bias correction were used to assess differences.
In both paired and unpaired sets of samples, a decreasing trend for α-diversity in semen after vasectomy was observed. Shannon diversity, the relative abundance of species with an abundance >2%, and composition were not significantly changed. Sphingomonas, Brevundimonas, and Paracoccus abundance decreased after vasectomy, while Corynebacterium abundance increased. The results may be limited by the sample size and lack of demographic heterogeneity.
Vasectomy is followed by a decrease in α-diversity and changes in the relative abundance of bacterial species in the semen microbiome. Further investigation is necessary to understand the clinical significance of these changes after vasectomy.
We evaluated changes in the bacteria species in semen after vasectomy. We found that vasectomy decreased the richness and evenness of bacteria species in semen, but the overall bacterial community remained similar. Further studies are needed to assess the implications of changes in semen bacteria after vasectomy.
摘要：
背景：在美国，每年大约有50万例输精管切除术。关于男性绝育对精液微生物组的影响以及是否会促使微生物群失调的文献很少。
目的：通过比较男性输精管结扎前后的精液样本，研究输精管结扎是否会引起精液微生物组的变化，如果输精管切除男性的精液微生物组分布在多样性和丰度方面遵循特定的模式。
方法：从2021年7月至2022年2月，我们在一家门诊诊所前瞻性收集并分析了58名男性的精液样本。18名男子在输精管切除术之前和之后3个月提供了精液样本。我们还在输精管切除术后3个月从22名可育的非输精管切除的男性和另外18名输精管切除的男性中收集了精液样本。
方法：精液微生物组α-多样性，β-多样性,和相对丰度最初在配对之间进行比较,然后在未配对的输精管切除和非输精管切除样品之间进行比较.方差分析(ANOVA)，置换多变量方差分析，并使用偏倚校正的微生物组成分析来评估差异。
结论：在配对和未配对的样品组中，输精管结扎术后精液中α-多样性呈下降趋势。香农多样性,丰度>2%的物种的相对丰度，组成无明显变化。鞘氨醇单胞菌,Brevundimonas,输精管结扎术后副球菌丰度下降，而棒状杆菌丰度增加。结果可能受到样本量和缺乏人口异质性的限制。
结论：输精管结扎后，精液微生物组中的α-多样性和细菌种类的相对丰度发生变化。需要进一步调查以了解输精管结扎术后这些变化的临床意义。
结果：我们评估了输精管结扎后精液中细菌种类的变化。我们发现输精管结扎术降低了精液中细菌种类的丰富度和均匀度，但总体细菌群落仍然相似。需要进一步的研究来评估输精管结扎术后精液细菌变化的影响。
目的：通过比较男性输精管结扎前后的精液样本，研究输精管结扎是否会引起精液微生物组的变化，
方法：从2021年7月至2022年2月，我们在一家门诊诊所前瞻性收集并分析了58名男性的精液样本。
方法：精液微生物组α-多样性，
结论：在配对和未配对的样品组中，
结论：输精管结扎后，精液微生物组中的α-多样性和细菌种类的相对丰度发生变化。
结果：我们评估了输精管结扎后精液中细菌种类的变化。
