Abstract：
Withania somnifera (W. somnifera) is a plant with remarkable pharmacological properties. The plant has an impressive profile of medicinal uses in the folk medicine system of several civilizations.
This comprehensive study is aimed to characterize phytochemicals in fruit of W. somnifera and tested for anticancer potential to find out active candidate in disease prevention and treatment.
The bioactive components from W. somn-ifera fruit were extracted with polar and non-polar solvents. Anticancer potential of the isolated bioactive was assessed against different cancer cell lines through MTT assay and Incucytes imaging analysis. The extracts were characterized for secondary metabolites using GC-MS (gas chromatography-mass spectrometer), LCMS (liquid chromatography-mass spectrometry)-ESI (electrospray Ionization) and 1H-NMR (electrospray Ionization) techniques.
Both freeze-dried and rotary evaporator con-densed extracts exhibited anticancer potential against MDA-MB-231, MCF7- SKOV3 and SKBR3 cell lines. The tested extracts have cell growth inhibition potential ag-ainst mammalian cancer cell line. Hexacosanedioic acid purified from -hexane extract through HPLC was inves-tigated for its cytotoxicity against breast cancer cell line SKBR3 by using Incucytes imaging analysis.
We found that a variety of bioactive compounds existed in this plant. One identified compound that was not investigated for cytotoxicity in previous studies was purified and its application showed cytotoxicity on breast cancer cell lines. A number of bioactive identified from fruit may have an effective potential for development into chemotherapy drugs.
This comprehensive study is aimed to characterize phytochemicals in fruit of W. somnifera and tested for anticancer potential to find out active candidate in disease prevention and treatment.
The bioactive components from W. somn-ifera fruit were extracted with polar and non-polar solvents. Anticancer potential of the isolated bioactive was assessed against different cancer cell lines through MTT assay and Incucytes imaging analysis. The extracts were characterized for secondary metabolites using GC-MS (gas chromatography-mass spectrometer), LCMS (liquid chromatography-mass spectrometry)-ESI (electrospray Ionization) and 1H-NMR (electrospray Ionization) techniques.
Both freeze-dried and rotary evaporator con-densed extracts exhibited anticancer potential against MDA-MB-231, MCF7- SKOV3 and SKBR3 cell lines. The tested extracts have cell growth inhibition potential ag-ainst mammalian cancer cell line. Hexacosanedioic acid purified from -hexane extract through HPLC was inves-tigated for its cytotoxicity against breast cancer cell line SKBR3 by using Incucytes imaging analysis.
We found that a variety of bioactive compounds existed in this plant. One identified compound that was not investigated for cytotoxicity in previous studies was purified and its application showed cytotoxicity on breast cancer cell lines. A number of bioactive identified from fruit may have an effective potential for development into chemotherapy drugs.
摘要：
有忧郁症（W.somnifera）是一种具有显着的药理特性的植物。该植物在几种文明的民间医学系统中具有令人印象深刻的药用特征。
这项全面的研究旨在表征W.somnifera果实中的植物化学物质，并测试抗癌潜力，以找出疾病预防和治疗中的活性候选物。
用极性和非极性溶剂提取W.somn-ifera果实中的生物活性成分。通过MTT测定和Incucytes成像分析，针对不同的癌细胞系评估了分离的生物活性物质的抗癌潜力。使用GC-MS（气相色谱-质谱仪）表征提取物的次级代谢产物，LCMS(液相色谱-质谱)-ESI(电喷雾电离)和1H-NMR(电喷雾电离)技术。
冷冻干燥和旋转蒸发仪浓缩的提取物均显示出对MDA-MB-231，MCF7-SKOV3和SKBR3细胞系的抗癌潜力。测试的提取物对哺乳动物癌细胞系具有细胞生长抑制潜力。通过使用Incucytes成像分析，研究了通过HPLC从-己烷提取物中纯化的六烷二酸对乳腺癌细胞系SKBR3的细胞毒性。
我们发现该植物中存在多种生物活性化合物。纯化了一种在先前研究中未研究细胞毒性的鉴定化合物，其应用显示了对乳腺癌细胞系的细胞毒性。从水果中鉴定出的许多生物活性物质可能具有开发成化疗药物的有效潜力。
这项全面的研究旨在表征W.somnifera果实中的植物化学物质，并测试抗癌潜力，以找出疾病预防和治疗中的活性候选物。
用极性和非极性溶剂提取W.somn-ifera果实中的生物活性成分。通过MTT测定和Incucytes成像分析，针对不同的癌细胞系评估了分离的生物活性物质的抗癌潜力。使用GC-MS（气相色谱-质谱仪）表征提取物的次级代谢产物，LCMS(液相色谱-质谱)-ESI(电喷雾电离)和1H-NMR(电喷雾电离)技术。
冷冻干燥和旋转蒸发仪浓缩的提取物均显示出对MDA-MB-231，MCF7-SKOV3和SKBR3细胞系的抗癌潜力。测试的提取物对哺乳动物癌细胞系具有细胞生长抑制潜力。通过使用Incucytes成像分析，研究了通过HPLC从-己烷提取物中纯化的六烷二酸对乳腺癌细胞系SKBR3的细胞毒性。
我们发现该植物中存在多种生物活性化合物。纯化了一种在先前研究中未研究细胞毒性的鉴定化合物，其应用显示了对乳腺癌细胞系的细胞毒性。从水果中鉴定出的许多生物活性物质可能具有开发成化疗药物的有效潜力。
