Abstract：
BACKGROUND: In vitro studies have produced conflicting results about the significance of the JC Polyoma Virus (JCV) in the human cancers.
OBJECTIVE: Our study aims to detect the presence of JCV Large T antigen (LTag) together with viral load quantitation in the prostate tumor samples to assess if JCV harbors risk factor for prostate cancer (PCa).
METHODS: This was a case control-based study. A total of 110 patients participated in this study, including 55 patients with PCa and another 55 patients with benign prostatic hyperplasia (BPH) as cases and controls, respectively. Tissue, blood and urine samples were collected from each participant. Tissues samples were analyzed for the presence of JCV Ltag using a direct immunofluorescence assay (IF). Only positive IF tested samples were subjected to viral quantitation assay. Data were collected and managed using SPSS version 20.
RESULTS: The JCV LTag in the cases group was 23.63% (13/55) which was higher than that of the controls group 5.45% (3/55) with a P. value of .006 and O.R of 5.76. The mean of viral load was significantly higher among cases tissue specimens 20156 ± 5450 copies/ml compared to controls group 6378 ± 2456copies/ml with P-value of .002. The virus was detected in 11/13 (84.6%) urine samples of cases with a mean viral load of 14068 ± 4590 copies/ml compared to 2/3 (66.6%) of controls viral load 2534 ± 1267 copies/ml.
CONCLUSIONS: In conclusion, a higher JCV LTag with more viral load were detected in cases group compared to controls. Our findings support a strong relationship between JCV infection and the probability of developing PCa.
OBJECTIVE: Our study aims to detect the presence of JCV Large T antigen (LTag) together with viral load quantitation in the prostate tumor samples to assess if JCV harbors risk factor for prostate cancer (PCa).
METHODS: This was a case control-based study. A total of 110 patients participated in this study, including 55 patients with PCa and another 55 patients with benign prostatic hyperplasia (BPH) as cases and controls, respectively. Tissue, blood and urine samples were collected from each participant. Tissues samples were analyzed for the presence of JCV Ltag using a direct immunofluorescence assay (IF). Only positive IF tested samples were subjected to viral quantitation assay. Data were collected and managed using SPSS version 20.
RESULTS: The JCV LTag in the cases group was 23.63% (13/55) which was higher than that of the controls group 5.45% (3/55) with a P. value of .006 and O.R of 5.76. The mean of viral load was significantly higher among cases tissue specimens 20156 ± 5450 copies/ml compared to controls group 6378 ± 2456copies/ml with P-value of .002. The virus was detected in 11/13 (84.6%) urine samples of cases with a mean viral load of 14068 ± 4590 copies/ml compared to 2/3 (66.6%) of controls viral load 2534 ± 1267 copies/ml.
CONCLUSIONS: In conclusion, a higher JCV LTag with more viral load were detected in cases group compared to controls. Our findings support a strong relationship between JCV infection and the probability of developing PCa.
摘要：
背景：关于JC多瘤病毒（JCV）在人类癌症中的重要性，体外研究产生了相互矛盾的结果。
目的：我们的研究旨在检测前列腺肿瘤样本中JCV大T抗原（LTag）的存在以及病毒载量定量，以评估JCV是否具有前列腺癌（PCa）的危险因素。
方法：这是一项基于病例对照的研究。共有110名患者参加了这项研究，包括55例PCa患者和另外55例良性前列腺增生（BPH）患者作为病例和对照，分别。组织,收集每位参与者的血液和尿液样本.使用直接免疫荧光测定(IF)分析组织样品中JCVL标签的存在。仅对阳性IF测试样品进行病毒定量测定。使用SPSS版本20收集和管理数据。
结果：病例组的JCVLTag为23.63％（13/55），高于对照组的5.45％（3/55），P值为.006，O.R为5.76。与对照组6378±2456copies/ml相比，病例组织标本20156±5450拷贝/ml的病毒载量平均值显着升高，P值为.002。在11/13（84.6％）例的尿液样本中检测到该病毒，平均病毒载量为14068±4590拷贝/ml，而对照病毒载量为2/3（66.6％）2534±1267拷贝/ml。
结论：结论：与对照组相比,在病例组中检测到更高的JCVLTag,病毒载量更高.我们的发现支持JCV感染与发生PCa的可能性之间的密切关系。
目的：我们的研究旨在检测前列腺肿瘤样本中JCV大T抗原（LTag）的存在以及病毒载量定量，以评估JCV是否具有前列腺癌（PCa）的危险因素。
方法：这是一项基于病例对照的研究。共有110名患者参加了这项研究，包括55例PCa患者和另外55例良性前列腺增生（BPH）患者作为病例和对照，分别。组织,收集每位参与者的血液和尿液样本.使用直接免疫荧光测定(IF)分析组织样品中JCVL标签的存在。仅对阳性IF测试样品进行病毒定量测定。使用SPSS版本20收集和管理数据。
结果：病例组的JCVLTag为23.63％（13/55），高于对照组的5.45％（3/55），P值为.006，O.R为5.76。与对照组6378±2456copies/ml相比，病例组织标本20156±5450拷贝/ml的病毒载量平均值显着升高，P值为.002。在11/13（84.6％）例的尿液样本中检测到该病毒，平均病毒载量为14068±4590拷贝/ml，而对照病毒载量为2/3（66.6％）2534±1267拷贝/ml。
结论：结论：与对照组相比,在病例组中检测到更高的JCVLTag,病毒载量更高.我们的发现支持JCV感染与发生PCa的可能性之间的密切关系。
