Abstract：
Quantitative real-time PCR and next-generation sequencing (NGS) are invaluable techniques to analyze T cell receptor (Tcr) gene rearrangements in mouse lymphocyte populations. Although these approaches are powerful, they also have limitations that must be accounted for in experimental design and data interpretation. Here, we provide relevant background required for understanding these limitations and then outline established quantitative real-time PCR and NGS methods that can be used for analysis of mouse Tcra and Tcrb gene rearrangements in mice.
摘要：
定量实时PCR和下一代测序（NGS）是分析小鼠淋巴细胞群体中T细胞受体（Tcr）基因重排的宝贵技术。虽然这些方法很强大，它们也有局限性,必须在实验设计和数据解释中加以考虑.这里,我们提供了理解这些局限性所需的相关背景，然后概述了已建立的定量实时PCR和NGS方法，这些方法可用于分析小鼠Tcra和Tcrb基因重排。
