Abstract：
Given the rising prevalence of lipid metabolic disorders and malignant diseases, we aimed to establish an in vivo hypercholesterinaemic tumour-bearing rat model for the induction and assessment of these conditions. A normal standard CRLT/N, 2 (baseline),- or 4 (2 + 2, pretreated)-week-long butter and cholesterol rich (BCR) diet was applied to mesoblastic nephroma (Ne/De) and myelomonoblastic leukaemia (My1/De) tumour-bearing and healthy control Long—Evans and Fischer 344 rats. The beginning of chow administration started in parallel with tumour induction and the 2 weeks of pre-transplantation in the baseline and pretreated groups, respectively. Fourteen days post-inoculation, the measurement of lipid parameters and [18F]F-FDG PET/MRI examinations was executed. The comparable lipid status of baseline healthy and tumorous rats proves that regardless of tumour presence, BCR-based hypercholesterolemia was achieved. A higher tumour mass among pretreated tumorous animals was found when compared to the control groups (p < 0.05, p < 0.01). Further, a visually greater [18F]F-FDG accumulation was observed in pretreated BCR tumorous animals; however, the quantitative data (SUVmean: 9.86 ± 0.98, 9.68 ± 1.24; SUVmax: 19.63 ± 1.20; 17.56 ± 3.21 for Ne/De and My1/De, respectively) were not statistically significantly different from those of the CRLT/N tumorous rats (SUVmean: 8.40 ± 1.42, 7.22 ± 1.06 and SUVmax: 15.99 ± 2.22, 12.46 ± 1.96 for control Ne/De and My1/De, respectively). Our model seems to be appropriate for simultaneously investigating hypercholesterolemia and cancer in the same rat.
摘要：
鉴于脂质代谢紊乱和恶性疾病的患病率上升，我们的目的是建立一种体内高胆固醇血症荷瘤大鼠模型，以诱导和评估这些疾病。正常标准CRLT/N，2（基线），-或4（22，预处理）周的黄油和富含胆固醇（BCR）的饮食被应用于中胚层肾瘤（Ne/De）和骨髓母细胞白血病（My1/De）肿瘤和健康对照Long-Evans和Fischer344大鼠。在基线和预处理组中，开始chow给药与肿瘤诱导和2周预移植平行开始，分别。接种后14天，进行脂质参数测量和[18F]F-FDGPET/MRI检查。基线健康大鼠和肿瘤大鼠的脂质状态相当，证明无论肿瘤的存在，实现了基于BCR的高胆固醇血症。与对照组相比，经预处理的肿瘤动物的肿瘤质量更高（p＆lt;0.05，p＆lt;0.01）。Further,在预处理的BCR肿瘤动物中观察到视觉上更大的[18F]F-FDG积累；然而，定量数据（对于Ne/De和My1/De，SUVmean：9.86±0.98，9.68±1.24；SUVmax：19.63±1.20；17.56±3.21，分别）与CRLT/N肿瘤大鼠的差异无统计学意义（对照Ne/De和My1/De的SUVmean：8.40±1.42、7.22±1.06和SUVmax：15.99±2.22、12.46±1.96，分别)。我们的模型似乎适用于同时研究同一只大鼠的高胆固醇血症和癌症。
