Abstract：
Scabies is a common parasitic dermatological infection worldwide that is often neglected. Scabies mites stimulate host inflammatory symptoms via secreted and excreted proteins, which induce basophil and mast cell degranulation and host histamine release. However, the mechanism of degranulation and histamine release is unclear. Moreover, the Sarcoptes scabiei translationally controlled tumor protein (TCTP) is predicted as an excreted protein, which may be involved in host inflammatory response regulation. First, we evaluated S. scabiei TCTP gene (SsTCTP) transcription in larvae, nymphs, and adults by qRT-PCR, and SsTCTP transcription was highest in larvae, followed by nymphs. Second, we found that the S. scabiei TCTP recombinant protein (rSsTCTP) promoted mice histamine release in vivo by Evans blue Miles assay. Therefore, to further explore the possible role of S. scabiei TCTP in host inflammatory response regulation, we established a degranulation model of KU812 cells. The results of the degranulation model suggested that rSsTCTP could induce enhanced degranulation of KU812 cells and increase the secretion of histamine and the expression of IL-4, IL-6, and IL-13 in vitro. In conclusion, we speculate that scabies mites could stimulate host histamine release and Th2 response by excreting S. scabiei TCTP.
摘要：
镰刀菌是世界范围内常见的寄生虫性皮肤病感染，经常被忽视。镰刀螨通过分泌和排泄的蛋白质刺激宿主炎症症状，诱导嗜碱性粒细胞和肥大细胞脱颗粒和宿主组胺释放。然而,脱颗粒和组胺释放的机制尚不清楚。此外，Sarcoptesscabiei翻译控制的肿瘤蛋白（TCTP）被预测为排泄蛋白，这可能与宿主炎症反应调节有关。首先,我们评估了S.scabieiTCTP基因（SsTCTP）在幼虫中的转录，若虫,和成人通过qRT-PCR，SsTCTP转录在幼虫中最高，其次是若虫。第二,我们发现S.scabieiTCTP重组蛋白（rSsTCTP）通过EvansblueMiles试验促进小鼠体内组胺释放。因此,为了进一步探讨S.scabieiTCTP在宿主炎症反应调节中的可能作用，建立KU812细胞脱颗粒模型。脱颗粒模型的结果表明，rSsTCTP可以在体外诱导KU812细胞增强脱颗粒，并增加组胺的分泌和IL-4，IL-6和IL-13的表达。总之,我们推测sc螨可以通过排泄S.scabieiTCTP刺激宿主组胺释放和Th2反应。
