Abstract：
Matrix Metalloproteinases (MMPs) have been found to have important roles in vascular pathology and may be involved in the occurrence of pre-eclampsia. In this study, the serum levels of MMP-2, -7, -9 in normal pregnant women and pre-eclampsia patients were analyzed to assess their predictive value.
A total of 1563 pregnant women from Peking University Third Hospital, from February 2021 to October 2021, were enrolled. Serum samples were collected from patients one to three times, during the different trimesters. Among the 102 singleton pre-eclampsia patients, we collected samples from 33 patients in the first trimester (6-13 GW), 33 in the second trimester (14-28 GW), 41 in the third trimester (29-41 GW) and 28 after onset of pre-eclampsia. Samples from each trimester were collected before the onset of pre-eclampsia. Then we selected 35, 37, 43 and 25 samples from 124 healthy pregnant women by matching their age, BMI and gestational weeks, using these as the control groups. Serum levels of MMP-2, -7, -9 were detected by ELISA. The receiver operating characteristic (ROC) curve was used to evaluate their predictive value.
Except for the first trimester, MMP-2 and MMP-7 were significantly higher in the pre-eclampsia group (p < 0.5). Additionally, in the pre-eclampsia group, MMP-9 increased significantly in the first trimester and after the onset of pre-eclampsia but decreased significantly in the second and third trimesters (p < 0.5). The ROC curve indicated that MMP-9, MMP-2 and MMP-7 were the best indicators for predicting pre-eclampsia in the first, second and third trimesters, respectively.
Increased MMP-2 and MMP-7 levels and a decreased MMP-9 level seem to be related to the pathogenesis of pre-eclampsia and are expected to be potential predictors of pre-eclampsia.
A total of 1563 pregnant women from Peking University Third Hospital, from February 2021 to October 2021, were enrolled. Serum samples were collected from patients one to three times, during the different trimesters. Among the 102 singleton pre-eclampsia patients, we collected samples from 33 patients in the first trimester (6-13 GW), 33 in the second trimester (14-28 GW), 41 in the third trimester (29-41 GW) and 28 after onset of pre-eclampsia. Samples from each trimester were collected before the onset of pre-eclampsia. Then we selected 35, 37, 43 and 25 samples from 124 healthy pregnant women by matching their age, BMI and gestational weeks, using these as the control groups. Serum levels of MMP-2, -7, -9 were detected by ELISA. The receiver operating characteristic (ROC) curve was used to evaluate their predictive value.
Except for the first trimester, MMP-2 and MMP-7 were significantly higher in the pre-eclampsia group (p < 0.5). Additionally, in the pre-eclampsia group, MMP-9 increased significantly in the first trimester and after the onset of pre-eclampsia but decreased significantly in the second and third trimesters (p < 0.5). The ROC curve indicated that MMP-9, MMP-2 and MMP-7 were the best indicators for predicting pre-eclampsia in the first, second and third trimesters, respectively.
Increased MMP-2 and MMP-7 levels and a decreased MMP-9 level seem to be related to the pathogenesis of pre-eclampsia and are expected to be potential predictors of pre-eclampsia.
摘要：
已发现基质金属蛋白酶（MMPs）在血管病理中具有重要作用，并可能参与先兆子痫的发生。在这项研究中,分析正常孕妇和子痫前期患者的血清MMP-2，-7，-9水平，以评估其预测价值。
北京大学第三医院共1563名孕妇，从2021年2月到2021年10月，都被录取了。从患者身上采集血清样本一至三次,在不同的三个月。在102名单胎先兆子痫患者中，我们收集了33例妊娠早期患者（6-13GW）的样本，33在中期（14-28GW），妊娠晚期41例（29-41GW）和先兆子痫发病后28例。在先兆子痫发作之前收集每个三个月的样品。然后我们从124名健康孕妇中选择35、37、43和25个样本，通过匹配他们的年龄，BMI和孕周，使用这些作为控制组。ELISA法检测血清MMP-2、-7、-9水平。使用受试者工作特征（ROC）曲线来评估其预测值。
除了孕早期,MMP-2和MMP-7在先兆子痫组中显著增高(p<0.5)。此外,在先兆子痫组，MMP-9在妊娠早期和先兆子痫发病后显著增加,但在妊娠中期和晚期显著降低(p<0.5)。ROC曲线显示MMP-9、MMP-2和MMP-7是预测先兆子痫的最佳指标,第二和第三个三个月，分别。
升高的MMP-2和MMP-7水平和降低的MMP-9水平似乎与先兆子痫的发病机制有关，并有望成为先兆子痫的潜在预测因子。
北京大学第三医院共1563名孕妇，从2021年2月到2021年10月，都被录取了。从患者身上采集血清样本一至三次,在不同的三个月。在102名单胎先兆子痫患者中，我们收集了33例妊娠早期患者（6-13GW）的样本，33在中期（14-28GW），妊娠晚期41例（29-41GW）和先兆子痫发病后28例。在先兆子痫发作之前收集每个三个月的样品。然后我们从124名健康孕妇中选择35、37、43和25个样本，通过匹配他们的年龄，BMI和孕周，使用这些作为控制组。ELISA法检测血清MMP-2、-7、-9水平。使用受试者工作特征（ROC）曲线来评估其预测值。
除了孕早期,MMP-2和MMP-7在先兆子痫组中显著增高(p<0.5)。此外,在先兆子痫组，MMP-9在妊娠早期和先兆子痫发病后显著增加,但在妊娠中期和晚期显著降低(p<0.5)。ROC曲线显示MMP-9、MMP-2和MMP-7是预测先兆子痫的最佳指标,第二和第三个三个月，分别。
升高的MMP-2和MMP-7水平和降低的MMP-9水平似乎与先兆子痫的发病机制有关，并有望成为先兆子痫的潜在预测因子。
