Abstract：
Hepatocellular carcinoma is the cancer with the highest incidence among liver cancers and how to treat this cancer effectively is still a difficult problem we must face. We selected meiotic nuclear divisions 1 (MND1) as the study object by combining data from The Cancer Genome Atlas (TCGA) database with prognostic survival analysis. We validated the value of MND1 in evaluating the prognosis of hepatocellular carcinoma through a diagnostic and prognostic model. At the same time, cellular experiments were used to demonstrate the effect of MND1 on hepatocellular carcinoma proliferation and migration. We used short hairpin RNA (shRNA) to knock down MND1 in Hun7 and HCCLM3 cell lines. Through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and colony formation assays, we found that knocking down MND1 reduced the proliferation of cells. Through wound healing and Transwell assays, we found that knocking down MND1 reduced cell migration and invasion. Moreover, we found that MND1 can promote the proliferation, migration, and invasion of Hep3B cells by overexpressing MND1. Therefore, in general, MND1 is expected to be a gene that can effectively diagnose and treat hepatocellular carcinoma.
摘要：
肝细胞癌是肝癌中发病率最高的癌症，如何有效治疗肝癌仍然是我们必须面对的难题。我们选择减数分裂核分裂1（MND1）作为研究对象，通过将来自癌症基因组图谱（TCGA）数据库的数据与预后生存分析相结合。我们通过诊断和预后模型验证了MND1在评估肝细胞癌预后中的价值。同时,细胞实验用于证明MND1对肝细胞癌增殖和迁移的影响。我们使用短发夹RNA(shRNA)敲低Hun7和HCCLM3细胞系中的MND1。通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物（MTT）和集落形成测定，我们发现敲低MND1会降低细胞的增殖。通过伤口愈合和Transwell分析，我们发现敲低MND1会降低细胞的迁移和侵袭。此外，我们发现MND1可以促进增殖，迁移,和过表达MND1对Hep3B细胞的侵袭。因此,总的来说,MND1有望成为能够有效诊断和治疗肝细胞癌的基因。
