Abstract：
This study focuses on developing a microarchitectural bilayer structure for stimulating the two top layers of skin tissue (epidermis and dermis) fabricated using a one-step freeze-drying method. Cellulose nanofibers (CNFs) and poly (vinyl) alcohol (PVA) were used as a biocompatible scaffolding material, and the composition was designed in such a way that it provides physical and biological property attributes. In this work, scaffolding materials with integrated layer structures and well interconnected and open pore structures were obtained. This bilayer structure had porosity with a pore size of 19.72 μm and 90.71 μm for the simulation of the epidermis and dermis, respectively. The production and expression of laminin, collagen IV, and keratin 10 proteins in the bilayer cryogel scaffolds obtained from the immunofluorescence study were 49.7 %, 63.8 %, and 49.3 %, respectively. The extracellular matrix (ECM) was produced in each scaffold layer. The observations confirmed that the porosity and pore size of both N1 and N2 layers were appropriate for the fibroblast and keratinocyte cells, respectively. H&E stained cross-sections of bilayer cryogel scaffolds illustrated epidermal and dermal layers produced by co-culturing keratinocytes and fibroblasts. Based on the results, the bilayer CNF/PVA scaffold can be used in skin tissue engineering applications. However, more experiments and in vivo evaluations are needed to express this conclusion more accurately.
摘要：
这项研究的重点是开发一种微建筑双层结构，用于刺激使用一步冷冻干燥方法制造的皮肤组织（表皮和真皮）的两个顶层。纤维素纳米纤维（CNFs）和聚乙烯醇（PVA）被用作生物相容性支架材料，组合物的设计方式使其具有物理和生物属性。在这项工作中,获得了具有集成层结构和良好互连和开孔结构的支架材料。这种双层结构具有孔隙度，孔径为19.72μm和90.71μm，用于模拟表皮和真皮，分别。层粘连蛋白的产生和表达，胶原蛋白IV，免疫荧光研究获得的双层冷冻凝胶支架中角蛋白10蛋白占49.7%，63.8%,49.3%，分别。在每个支架层中产生细胞外基质(ECM)。观察结果证实，N1和N2层的孔隙率和孔径大小适合成纤维细胞和角质形成细胞，分别。双层冷冻凝胶支架的H&E染色的横截面示出了通过共培养角质形成细胞和成纤维细胞产生的表皮层和真皮层。根据结果,双层CNF/PVA支架可用于皮肤组织工程应用。然而,需要更多的实验和体内评估来更准确地表达这一结论。
