Abstract：
Tissue regeneration is the ultimate treatment for many degenerative diseases, however, repair and regeneration of damaged organs or tissues remains a challenge. Previously, we showed that B1 Ab and H3 Ab induce stem cells to differentiate into microglia and brown adipocyte-like cells, while trafficking to the brain and heart, respectively. Here, we present data showing that another selected agonist antibody, P1 antibody, induces the migration of cells to the pancreatic islets and differentiates human stem cells into beta-like cells. Interestingly, our results suggest the purified P1 Ab induces beta-like cells from fresh, human CD34+ hematopoietic stem cells and mouse bone marrow. In addition, stem cells with P1 Ab bound to expressed periostin (POSTN), an extracellular matrix protein that regulates tissue remodeling, selectively migrate to mouse pancreatic islets. Thus, these results confirm that our in vivo selection system can be used to identify antibodies from our library which are capable of inducing stem cell differentiation and cell migration to select tissues for the purpose of regenerating and remodeling damaged organ systems.
摘要：
组织再生是许多退行性疾病的最终治疗方法，然而,受损器官或组织的修复和再生仍然是一个挑战。以前,我们发现B1Ab和H3Ab诱导干细胞分化为小胶质细胞和棕色脂肪细胞样细胞，当贩卖到大脑和心脏时，分别。这里,我们提供的数据显示另一种选择的激动剂抗体，P1抗体，诱导细胞向胰岛迁移并将人干细胞分化为β样细胞。有趣的是,我们的结果表明，纯化的P1Ab诱导新鲜的β样细胞，人CD34+造血干细胞和小鼠骨髓。此外,具有P1Ab与表达骨膜素（POSTN）结合的干细胞，一种调节组织重塑的细胞外基质蛋白，选择性迁移到小鼠胰岛。因此，这些结果证实，我们的体内选择系统可用于从我们的文库中鉴定能够诱导干细胞分化和细胞迁移的抗体，以选择组织以再生和重塑受损器官系统。
