PDF(Pubmed)
Abstract：
Zwitterionic sulfobetaine-based monolithic stationary phases have attracted increasing attention for their use in hydrophilic interaction chromatography. In this study, a novel hydrophilic polymeric monolith was fabricated through photo-initiated copolymerization of 3-(3-vinyl-1-imidazolio)-1-propanesulfonate (SBVI) with pentaerythritol triacrylate using methanol and tetrahydrofuran as the porogenic system. Notably, the duration for the preparation of this novel monolith was as little as 5 min, which was significantly shorter than that required for previously reported sulfobetaine-based monoliths prepared via conventional thermally initiated copolymerization. Moreover, these monoliths showed good morphology, permeability, porosity (62.4%), mechanical strength (over 15 MPa), column efficiency (51,230 plates/m), and reproducibility (relative standard deviations for all analytes were lower than 4.6%). Mechanistic studies indicated that strong hydrophilic and negative electrostatic interactions might be responsible for the retention of polar analytes on the zwitterionic SBVI-based monolith. In particular, the resulting monolith exhibited good anti-protein adhesion ability and low nonspecific protein adsorption. These excellent features seem to favor its application in bioanalysis. Therefore, the novel zwitterionic sulfobetaine-based monolith was successfully employed for the highly selective separation of small bioactive compounds and the efficient enrichment of N-glycopeptides from complex samples. In this study, we prepared a novel zwitterionic sulfobetaine-based monolith with good performance and developed a simpler and faster method for preparation of zwitterionic monoliths.
摘要：
基于两性离子的磺基甜菜碱的整体固定相因其在亲水相互作用色谱中的用途而引起了越来越多的关注。在这项研究中,以甲醇和四氢呋喃为致孔体系，通过3-（3-乙烯基-1-咪唑啉）-1-丙磺酸盐（SBVI）与季戊四醇三丙烯酸酯的光引发共聚制备了一种新型的亲水性聚合物整料。值得注意的是,这种新型整体材料的制备时间仅为5分钟，明显短于以前报道的通过常规热引发共聚制备的基于磺基甜菜碱的整料所需的时间。此外，这些巨石表现出良好的形态，渗透性,孔隙度（62.4%），机械强度（超过15MPa），色谱柱效率（51,230板/m），和重现性（所有分析物的相对标准偏差均低于4.6％）。机理研究表明，强烈的亲水和负静电相互作用可能是极性分析物在两性离子SBVI基整料上保留的原因。特别是,所得单体表现出良好的抗蛋白质粘附能力和低的非特异性蛋白质吸附。这些优异的特性似乎有利于其在生物分析中的应用。因此,新型两性离子磺基甜菜碱整料已成功用于高度选择性分离小的生物活性化合物和从复杂样品中有效富集N-糖肽。在这项研究中,我们制备了一种性能良好的新型两性离子磺基甜菜碱整料，并开发了一种更简单，更快捷的制备两性离子整料的方法。
