Abstract：
BACKGROUND: Proliferation of embryonic fibroblasts under the same cell culture conditions, hinny embryonic fibroblasts (HiEFs) was slower than horse embryonic fibroblast (HEFs), donkey embryonic fibroblasts (DEFs) and mule embryonic fibroblasts (MuEFs). The imprinted genes IGF2 and IGF2R are important for cell proliferation. Therefore, we investigated whether the slower proliferation of HiEFs is related to an aberrant gene expression of IGF2 or its receptors or genes influencing the expression of the IGF2 system.
RESULTS: Real-time polymerase chain reaction, immunofluorescence and cell starving experiment in HEFs, DEFs, MuEFs and HiEFs revealed that the slower proliferation of HiEF in vitro was related to its lower expression of IGF2R (P < 0.001). Moreover, quantification of allele-specific expression and bisulfate assay confirmed that in both MuEFs and HiEFs, IGF2R had normal maternal imprinting, implying that the imprint aberrant was not involved in the lower IGF2R expression in HiEFs.
CONCLUSIONS: The reduction of IGF2R expression in HiEFs is associated with its slower proliferation in vitro.
RESULTS: Real-time polymerase chain reaction, immunofluorescence and cell starving experiment in HEFs, DEFs, MuEFs and HiEFs revealed that the slower proliferation of HiEF in vitro was related to its lower expression of IGF2R (P < 0.001). Moreover, quantification of allele-specific expression and bisulfate assay confirmed that in both MuEFs and HiEFs, IGF2R had normal maternal imprinting, implying that the imprint aberrant was not involved in the lower IGF2R expression in HiEFs.
CONCLUSIONS: The reduction of IGF2R expression in HiEFs is associated with its slower proliferation in vitro.
摘要：
背景：在相同的细胞培养条件下，胚胎成纤维细胞的增殖，海马胚胎成纤维细胞（HiEF）比马胚胎成纤维细胞（HEFs）慢，驴胚胎成纤维细胞(DEFs)和骡子胚胎成纤维细胞(MuEFs)。印迹基因IGF2和IGF2R对细胞增殖具有重要意义。因此,我们研究了HiEF的缓慢增殖是否与IGF2或其受体的异常基因表达或影响IGF2系统表达的基因有关。
结果：实时聚合酶链反应，HEF的免疫荧光和细胞饥饿实验，DEF,MuEFs和HiEFs显示HiEF在体外的增殖较慢与其IGF2R的表达较低有关(P<0.001)。此外，等位基因特异性表达和硫酸氢盐测定的定量证实，在MuEF和HiEF中，IGF2R有正常的母体印记，这意味着印记异常与HiEF中较低的IGF2R表达无关。
结论：HiEF中IGF2R表达的减少与其体外增殖较慢有关。
结果：实时聚合酶链反应，HEF的免疫荧光和细胞饥饿实验，DEF,MuEFs和HiEFs显示HiEF在体外的增殖较慢与其IGF2R的表达较低有关(P<0.001)。此外，等位基因特异性表达和硫酸氢盐测定的定量证实，在MuEF和HiEF中，IGF2R有正常的母体印记，这意味着印记异常与HiEF中较低的IGF2R表达无关。
结论：HiEF中IGF2R表达的减少与其体外增殖较慢有关。
