RESULTS: Real-time polymerase chain reaction, immunofluorescence and cell starving experiment in HEFs, DEFs, MuEFs and HiEFs revealed that the slower proliferation of HiEF in vitro was related to its lower expression of IGF2R (P < 0.001). Moreover, quantification of allele-specific expression and bisulfate assay confirmed that in both MuEFs and HiEFs, IGF2R had normal maternal imprinting, implying that the imprint aberrant was not involved in the lower IGF2R expression in HiEFs.
CONCLUSIONS: The reduction of IGF2R expression in HiEFs is associated with its slower proliferation in vitro.
结果:实时聚合酶链反应,HEF的免疫荧光和细胞饥饿实验,DEF,MuEFs和HiEFs显示HiEF在体外的增殖较慢与其IGF2R的表达较低有关(P<0.001)。此外,等位基因特异性表达和硫酸氢盐测定的定量证实,在MuEF和HiEF中,IGF2R有正常的母体印记,这意味着印记异常与HiEF中较低的IGF2R表达无关。
结论:HiEF中IGF2R表达的减少与其体外增殖较慢有关。