PDF(Pubmed)
Abstract：
Clonorchiasis caused by Clonorchis sinensis is a mainly foodborne parasitic disease. It can lead to hepatobiliary duct inflammation, fibrosis, obstructive jaundice, liver cirrhosis, and even cholangiocarcinoma. Interleukin (IL)-10 is an immune-regulatory cytokine which plays an immunosuppressive role during infection. Our previous study found that IL-10 was increased in mice with C. sinensis infection. However, the role and mechanism of IL-10 playing in hepatobiliary injury induced by C. sinensis infection remain unknown. Herein, Il10+/+ mice and Il10+/- C57BL/6J mice were infected with C. sinensis. It was found that IL-10 deficiency aggravated biliary hyperplasia and exacerbated periductal fibrosis induced by C. sinensis infection. Moreover, IL-10 deficiency increased CD4+T cells and CD8+T cells but not macrophages in the liver of mice with infection. There were no apparent differences in Th1 and Treg cells between Il10+/+ and Il10+/- mice infected with C. sinensis. However, the proportion of Th17 cells in CD4+T cells in Il10+/- infected mice was significantly higher than that in Il10+/+ infected mice. IL-10 deficiency also enhanced the increase of Th17 cells induced by ESPs stimulation in vitro. Taken together, our results suggest that IL-10 plays a protective role in hepatobiliary injury in C57BL/6J mice induced by C. sinensis infection via inhibiting Th17 cells, which could deepen our understanding of the immunopathology of clonorchiasis.
摘要：
华支睾吸虫引起的华支睾吸虫病是一种主要的食源性寄生虫病。会导致肝胆管炎症,纤维化，阻塞性黄疸,肝硬化,甚至是胆管癌.白细胞介素(IL)-10是在感染期间发挥免疫抑制作用的免疫调节细胞因子。我们先前的研究发现，感染C.sinensis的小鼠中IL-10升高。然而,IL-10在中华绒螯蟹感染引起的肝胆损伤中的作用和机制尚不清楚。在这里，Il10+/+小鼠和Il10+/-C57BL/6J小鼠被感染。发现IL-10缺乏加重了中华梭菌感染引起的胆管增生和导管周围纤维化。此外，IL-10缺乏增加感染小鼠肝脏中的CD4T细胞和CD8T细胞，但不增加巨噬细胞。在感染中华绒螯蟹的Il10+/+和Il10+/-小鼠之间,Th1和Treg细胞没有明显差异。然而,Il10+/-感染小鼠CD4+T细胞中Th17细胞的比例明显高于Il10+/+感染小鼠。IL-10缺乏还增强了体外ESPs刺激诱导的Th17细胞的增加。一起来看,我们的结果表明，IL-10通过抑制Th17细胞在C57BL/6J小鼠肝胆损伤中起保护作用。这可以加深我们对华支睾吸虫病免疫病理学的理解。
