Abstract：
Selective determination of 4-aminobenzoic acid (ABA) in pharmaceutical and human serum samples was performed by molecularly imprinted polypyrrole and ultraviolet (UV) spectrophotometry, based on precipitation polymerization. The molecularly imprinted polymer (MIP) was prepared using pyrrole functional monomer and ABA template molecules. The significant factors controlling the performance of the synthesized ABAMIP sorbent were screened and optimized using Plackett- Burman design (PBD) and central composite design (CCD), respectively. The model was used to obtain the optimal values of the significant response factors. The predicted MIP to NIP response ratio demonstrated an approximate deviation of 5 % from the experimental value. Under the obtained optimal conditions, the calibration curve showed a linear range of 0.05-2 mM with a correlation coefficient (r2) of 0.9920 and a limit of detection (LOD) of 0.0310 mM. The method recovery for the analyte was obtained 88.10-100.5 in the investigated real samples. The proposed ABA-MIP sorbent showed an acceptable selectivity in the presence of some pharmaceuticals.
摘要：
通过分子印迹聚吡咯和紫外(UV)分光光度法选择性测定药物和人血清样品中的4-氨基苯甲酸(ABA),基于沉淀聚合。利用吡咯功能单体和ABA模板分子制备了分子印迹聚合物(MIP)。使用Placket-Burman设计（PBD）和中央复合设计（CCD）筛选和优化了控制合成ABAMIP吸附剂性能的重要因素，分别。该模型用于获得显著响应因子的最优值。预测的MIP与NIP响应比表明与实验值的大约5%的偏差。在获得的最优条件下，校准曲线显示线性范围为0.05-2mM,相关系数(r2)为0.9920,检测限(LOD)为0.0310mM.在所研究的实际样品中，分析物的方法回收率为88.10-100.5。所提出的ABA-MIP吸附剂在一些药物存在下显示出可接受的选择性。
