Abstract：
UNASSIGNED: Oral submucous fibrosis (OSMF) is a potentially malignant disorder associated with habit of chewing betel quid containing arecanut. Morphological features of OSMF especially fibrosis suggests a possibility of the hypoxic environment in diseased tissues. The adaptation of cells to hypoxia appears to be mediated via hypoxia inducible factor-1α (HIF-1α) which is also said to be associated with malignant transformation of epithelial cells in various other carcinomas like prostate and cervical carcinoma. Therefore, the main objective of this study was to investigate the role of HIF-1α in progression and malignant transformation of OSMF.
UNASSIGNED: The study group consisted of histo-pathologically diagnosed 30 cases of oral submucous fibrosis and 10 cases of OSCC were taken as control. The immunohistochemistry was carried out on neutral buffered formalin-fixed paraffin-embedded tissue sections by using the monoclonal antibody of HIF-1α. Statistical analysis was done using SPSS software version 2.0.
UNASSIGNED: A gradual and significant rise in the expression of HIF-1α was observed in various grades of OSMF and OSCC cases. HIF 1α expression was increased in cases showing hylanization and constricted blood vessels. A cut off value of 39.6% of HIF-1α positive cells was determined statistically to categorize the cases into high risk and low risk group for malignant transformation.
UNASSIGNED: Overexpression of HIF-1α may contribute to the progression and malignant transformation of OSMF. Cases expressing more than 40% of HIF-1α positive cells are at a greater risk for malignant transformation.
UNASSIGNED: The study group consisted of histo-pathologically diagnosed 30 cases of oral submucous fibrosis and 10 cases of OSCC were taken as control. The immunohistochemistry was carried out on neutral buffered formalin-fixed paraffin-embedded tissue sections by using the monoclonal antibody of HIF-1α. Statistical analysis was done using SPSS software version 2.0.
UNASSIGNED: A gradual and significant rise in the expression of HIF-1α was observed in various grades of OSMF and OSCC cases. HIF 1α expression was increased in cases showing hylanization and constricted blood vessels. A cut off value of 39.6% of HIF-1α positive cells was determined statistically to categorize the cases into high risk and low risk group for malignant transformation.
UNASSIGNED: Overexpression of HIF-1α may contribute to the progression and malignant transformation of OSMF. Cases expressing more than 40% of HIF-1α positive cells are at a greater risk for malignant transformation.
摘要：
未经证实：口腔粘膜下纤维化（OSMF）是一种潜在的恶性疾病，与咀嚼含有槟榔的槟榔有关。OSMF的形态特征尤其是纤维化表明患病组织中缺氧环境的可能性。细胞对缺氧的适应似乎是通过缺氧诱导因子-1α（HIF-1α）介导的，据说HIF-1α也与各种其他癌症如前列腺癌和宫颈癌中上皮细胞的恶性转化有关。因此,本研究的主要目的是探讨HIF-1α在OSMF进展和恶变中的作用。
UNASSIGNED：研究组以病理确诊的口腔粘膜下纤维化30例，OSCC10例作为对照。通过使用HIF-1α的单克隆抗体在中性缓冲的福尔马林固定的石蜡包埋的组织切片上进行免疫组织化学。使用SPSS软件2.0版进行统计学分析。
UNASSIGNED：在各种级别的OSMF和OSCC病例中观察到HIF-1α的表达逐渐显着升高。HIF1α的表达在显示轴突化和血管收缩的情况下增加。在统计学上确定39.6％的HIF-1α阳性细胞的临界值，以将病例分为恶性转化的高风险和低风险组。
未经证实：HIF-1α过表达可能导致OSMF的进展和恶性转化。表达超过40%的HIF-1α阳性细胞的病例具有更大的恶性转化风险。
UNASSIGNED：研究组以病理确诊的口腔粘膜下纤维化30例，OSCC10例作为对照。通过使用HIF-1α的单克隆抗体在中性缓冲的福尔马林固定的石蜡包埋的组织切片上进行免疫组织化学。使用SPSS软件2.0版进行统计学分析。
UNASSIGNED：在各种级别的OSMF和OSCC病例中观察到HIF-1α的表达逐渐显着升高。HIF1α的表达在显示轴突化和血管收缩的情况下增加。在统计学上确定39.6％的HIF-1α阳性细胞的临界值，以将病例分为恶性转化的高风险和低风险组。
未经证实：HIF-1α过表达可能导致OSMF的进展和恶性转化。表达超过40%的HIF-1α阳性细胞的病例具有更大的恶性转化风险。
