Abstract：
The enteric morphology, enteric microbiota structure and serum metabolomics of M. salmoides before and after infected by A. hydrophila were analysed to explore the pathogenic mechanism of A. hydrophila infection in M. salmoides. The results revealed that, after the infection of A. hydrophila, the villus boundary of largemouth bass became less obvious; the relative abundance of Proteobacteria and decreasing relative abundance of Tenericutes were increasing; genera relative abundance of putatively beneficial bacteria (Mycoplasma) were decreasing, whereas the genus Aeromonas increased after infection; serum metabolomic analysis showed that infection with A. hydrophila caused disorder to the metabolic processes of largemouth bass, particularly amino acid metabolism, and caused inflammation; several potential pathogen infection-related and significantly differential intestinal microbiota-related metabolite markers were identified, such as 6-hydroxy-5-methoxyindole glucuronide, zalcitabine, bilirubin, aciclovir. This study may provide new insights into the potential association between enteric microbiota and serum metabolism and the pathogenic mechanism of M. salmoides infected by A. hydrophila, providing a scientific basis for disease control in largemouth bass breeding.
摘要：
肠形态，分析了嗜水菌感染前后的肠道菌群结构和血清代谢组学，以探讨嗜水菌感染的致病机制。结果显示,感染嗜水气单胞菌后，大嘴鲈鱼的绒毛边界变得不那么明显。变形杆菌的相对丰度和Tenericutes的相对丰度正在增加；推定有益菌（支原体）的属相对丰度正在降低。而气单胞菌属感染后增加；血清代谢组学分析显示，嗜水气单胞菌感染导致大口鲈鱼的代谢过程紊乱，特别是氨基酸代谢，并引起炎症;几个潜在的病原体感染相关和显著差异肠道微生物群相关的代谢标志物被鉴定，如6-羟基-5-甲氧基吲哚葡糖苷酸，扎西他滨,胆红素,阿昔洛韦.这项研究可能为肠道菌群与血清代谢之间的潜在关联以及嗜水菌感染的致病机制提供新的见解。为大口鲈鱼养殖病害防治提供科学依据。
