Abstract：
Polycomb group (PcG) repressors and Trithorax group (TrxG) activators of transcription are essential for the proper development and maintenance of gene expression profiles in multicellular organisms. In Drosophila, PcG/TrxG proteins interact with DNA elements called PRE (Polycomb response elements). We have previously shown that the repressive activity of inactive PRE in transgenes can be induced by architectural protein-binding sites. It was shown that the induction of repression is associated with the recruitment of PcG/TrxG proteins, including the DNA-binding factors Pho and Combgap. In the present study, we tested the association of the two other PRE DNA-binding factors, GAF and Psq, with bxdPRE in the presence and absence of sites for architectural proteins. As a result, it was shown that both factors can be efficiently recruited to the bxdPRE only in the presence of adjacent binding sites for architectural proteins Su(Hw), CTCF, or Pita.
摘要：
转录的Polycomb组（PcG）阻遏物和Trithorax组（TrxG）激活物对于多细胞生物中基因表达谱的正确发展和维持至关重要。在果蝇中,PcG/TrxG蛋白与称为PRE(Polycomb响应元件)的DNA元件相互作用。我们先前已经表明，无活性的PRE在转基因中的抑制活性可以由建筑蛋白结合位点诱导。研究表明，抑制的诱导与PcG/TrxG蛋白的募集有关，包括DNA结合因子Pho和Combgap。在本研究中,我们测试了另外两种PREDNA结合因子的关联，GAF和PSQ，在存在和不存在建筑蛋白位点的情况下使用bxdPRE。因此,研究表明，只有在存在结构蛋白Su（Hw）的相邻结合位点的情况下，这两个因子才能有效地募集到bxdPRE，CTCF,或者Pita.
