Abstract：
(1) Background: Gastric cancer (GC) is the fourth leading cause of cancer death worldwide. Silver nanoparticles (Ag-NPs) have been increasingly used in the diagnosis and treatment of cancer due to their physicochemical properties. This study investigated the role of a kind of biosynthetic silver nanoparticle (b-Ag) in the development of GC, the enhancement of 5-fluorouracil (5F), and its mechanism. (2) Methods: X-ray, transmission electron microscopy (TEM), and UV absorbance were used to detect the characterizations of AgNPs. CCK8, Colony formation and a Transwell assay were performed to confirm the malignant behaviors of GC. DCFH-DA and DHE were used to detect intracellular reactive oxygen species (ROS). Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the mRNA expression of apoptosis-related genes. (3) Results: Compared with the chemosynthetic silver nanoparticles (c-Ag), b-Ag had a stronger cytokilling effect, and it had a better inhibition on the malignant phenotype of GC when combined with 5F. The b-Ag increased the expression of Bax and P53 while decreasing the expression of Bcl2. It also promoted the generation of intracellular ROS. (4) Conclusions: By promoting cell apoptosis and increasing intracellular ROS, b-Ag inhibited the development of GC and enhanced the inhibition of 5F on GC.
摘要：
(1)研究背景：胃癌(GC)是全球癌症死亡的第四大原因。银纳米粒子(Ag-NP)由于其物理化学性质而越来越多地用于癌症的诊断和治疗。本研究探讨了一种生物合成银纳米粒子(b-Ag)在气相色谱发展中的作用,增强5-氟尿嘧啶（5F），及其机制。（2）方法：X射线，透射电子显微镜（TEM），和UV吸光度用于检测AgNP的表征。进行CCK8，集落形成和Transwell测定以确认GC的恶性行为。DCFH-DA和DHE用于检测细胞内活性氧（ROS）。采用定量逆转录聚合酶链反应(qRT-PCR)检测凋亡相关基因的mRNA表达。（3）结果：与化学合成的银纳米颗粒（c-Ag）相比，b-Ag具有更强的细胞杀伤作用，与5F联用对GC的恶性表型有较好的抑制作用。b-Ag增加Bax和P53的表达，同时降低Bcl2的表达。它还促进了细胞内ROS的产生。(4)结论:通过促进细胞凋亡和增加细胞内ROS,b-Ag抑制了GC的发展，增强了5F对GC的抑制作用。
